The Effect of Thiotriazoline on Energy Production in Conditions of Chronic Myocardial Ischemia

Кастанаян, А. А.; Kartashova, Elena; Железняк, Е. И.

doi:10.21886/2712-8156-2020-1-1-84-90

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…In addition, we suggest that Thiotriazoline itself can be a NO carrier, forming stable S-nitrosyl complexes with it [59]. Thiotriazoline exhibits a cardioprotective effect, positively affecting energy metabolism in ischemic myocardium, increases ATP during ischemia and hypoxia due to the normalization of the Krebs cycle, increases the utilization of glucose and free fatty acids, and activates the conversion of lactate to pyruvate [60]. Thiotriazoline is also known to exhibit a cardioprotective effect and to increase the endurance of animals under working hypoxia by increasing HIF-1 and preserving mitochondrial ultrastructure.…”

Section: Discussionmentioning

confidence: 92%

Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy

Popazova,

Belenichev,

Yadlovskyi

et al. 2023

CIMB

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Many children and adults who have suffered prenatal hypoxia at an early age develop many serious diseases. This disease is an actual problem of pediatric cardiology and little studied. The aim was to analyze the cardioprotective effect of L-arginine, Thiotriazoline, Angioline, and Mildronate on the cardiovascular system of rats after prenatal hypoxia. Methods: The experiments were carried out on 50 female white rats; intraperitoneal sodium nitrite solution was administered daily to pregnant female rats after 16 days at a dose of 50 mg/kg. Control pregnant rats received saline. The offspring were divided into groups: 1—intact; 2—the control group of rat pups after PH, treated daily with physiological saline; 3—six groups of rat pups after PH, treated daily from the 1st to the 30th day after birth. Heat shock protein HSP70 was determined by enzyme immunoassay, ST2 Nitrotyrosine, and eNOS was observed by ELISA. Results: Angiolin showed a high cardioprotective effect even a month after discontinuation of the drug, and after introduction, the highest decrease in ST2 nitrotyrosine was revealed. Thiotriazoline and L-arginine have an antioxidant effect and a positive effect on eNOS expression, increasing the concentration of HSP70. Mildronate increased the expression of eNOS and the concentration of HSP70 in the blood of experimental rats after a course of administration, but did not show an antioxidant effect and did not reduce the concentration of nitrotyrosine. The results obtained indicate the cardioprotective effect of modulators of the NO system with different mechanisms of action of drugs after prenatal hypoxia.

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Section: Discussionmentioning

confidence: 92%

Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy

Popazova,

Belenichev,

Yadlovskyi

et al. 2023

CIMB

View full text Add to dashboard Cite

show abstract

“…The pharmacological effect of the combination is due to a positive effect on the synthesis, transport, and bioavailability of NO and physiological functions of this molecular messenger [44]. In rats in which isadrin-pituitrin myocardial infarction was modeled, Thiotriazoline stimulated LDH in the direction of the formation of pyruvate from lactate, which eliminated lactic acidosis and normalized intracellular pH, and stimulated the Krebs cycle by increasing pyruvate [45]. In the same experimental mode, Thiotriazoline activated the malate-aspartate shunt in the myocardium in the acute period of myocardial infarction.…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective Activity of Pharmacological Agents Affecting NO Production and Bioavailability in the Early Postnatal Period after Intrauterine Hypoxia in Rats

Popazova,

Belenichev,

Bukhtiyarova

et al. 2023

Biomedicines

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Intrauterine hypoxia in newborns leads to a multifaceted array of alterations that exert a detrimental impact on the cardiovascular system. The aim of this research was to assess the cardioprotective effects of modulators of the nitric oxide (NO) system, including L-arginine, Thiotriazoline, Angiolin, and Mildronate, during the early postnatal period following intrauterine hypoxia. Methods: The study involved 50 female white rats. Pregnant female rats were given a daily intraperitoneal dose of 50 mg/kg of sodium nitrite starting on the 16th day of pregnancy. A control group of pregnant rats received saline instead. The resulting offspring were divided into the following groups: Group 1—intact rats; Group 2—rat pups subjected to prenatal hypoxia (PH) and daily treated with physiological saline; and Groups 3 to 6—rat pups exposed to prenatal hypoxia and treated daily from the 1st to the 30th day after birth. Nitrotyrosine levels, eNOS, iNOS, and NO metabolites were evaluated using ELISA; to measure the expression levels of iNOS mRNA and eNOS mRNA, a PCR test was utilized. Results: Angiolin enhances the expression of eNOS mRNA and boosts eNOS activity in the myocardium of rats with ischemic conditions. Arginine and particularly Thiotriazoline exhibited a consistent impact in restoring normal parameters of the cardiac nitroxidergic system following PH. Mildronate notably raised iNOS mRNA levels and notably reduced nitrotyrosine levels, providing further support for its antioxidative characteristics.

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“…Thiotriazoline (morpholinium 3-methyl-1,2,4-triazolyl-5thioacet) is a specific scavenger of NO and its cytotoxic forms, increases the bioavailability of NO, protecting it from ROS. Thiotriazoline under conditions of ischemia enhances the compensatory activation of the malate-aspartate shuttle mechanism, reduces the inhibition of oxidation processes in the Krebs cycle while maintaining the intracellular ATP pool, exhibits hepato-, cardioprotective, anti-ischemic and antioxidant properties [10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Altered Blood Molecular Markers of Cardiovascular Function in Rats After Intrauterine Hypoxia and Drug Therapy

Popazova,

Belenichev,

Yadlovskyi

et al. 2023

Preprint

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Many children and adults who have suffered prenatal hypoxia at an early age develop many serious diseases. This disease is an actual problem of pediatric cardiology and little studied. The aim was to analyze the cardioprotective effect of L-arginine, Thiotriazoline, Angioline and Mildronate on the cardiovascular system of rats after prenatal hypoxia. Methods: The experiments were carried out on 50 female white rats; intraperitoneal sodium nitrite solution was administered daily to pregnant female rats after 16 days at a dose of 50 mg/kg. Control pregnant rats received saline. The offspring were divided into groups: 1 – intact; 2 – the control group of rat pups after PH, daily treated with physiological saline; 3 – 6 groups of rat pups after PH, treated daily from the 1st to the 30th day after birth. Heat shock protein HSP70 was determined by enzyme immunoassay, ST2 Nitrotyrosine, eNOS was observed by ELISA. Results: Angiolin showed a high cardioprotective effect even a month after discontinuation of the drug, and after introduction, the highest decrease in ST2, nitrotyrosine was revealed. Thiotriazoline and L-arginine have an antioxidant effect and a positive effect on eNOS expression. increased the concentration of HSP70. Mildronate increased the expression of eNOS and the concentration of HSP70 in the blood of experimental rats after a course of administration, but did not show an antioxidant effect and did not reduce the concentration of nitrotyrosine. The results obtained indicate the cardioprotective effect of modulators of the NO system with different mechanisms of action of drugs after experienced prenatal hypoxia.

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The Effect of Thiotriazoline on Energy Production in Conditions of Chronic Myocardial Ischemia

Cited by 3 publications

References 4 publications

Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy

Altered Blood Molecular Markers of Cardiovascular Function in Rats after Intrauterine Hypoxia and Drug Therapy

Cardioprotective Activity of Pharmacological Agents Affecting NO Production and Bioavailability in the Early Postnatal Period after Intrauterine Hypoxia in Rats

Altered Blood Molecular Markers of Cardiovascular Function in Rats After Intrauterine Hypoxia and Drug Therapy

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