The Effect of Thrombin Activation of Platelet-Rich Plasma on Demineralized Bone Matrix Osteoinductivity

Abstract

Platelet-rich plasma significantly increased in vivo demineralized bone matrix osteoinductivity only when used without thrombin activation.

Treatment of osteochondral defects of the talus

“…Th is type of DBM has shown consistent composition and proven eff ectiveness in previous studies using a heterotopic bone formation model and an in vitro model. 173,317,318 Possibly, the natural circumstances of the joint (i.e., high loading forces and possible intrusion of fl uid into the OCD) might preclude the eff ectiveness of the DBM in the present study. Remnants of the DBM were found in two specimens, suggesting not all the material had remodeled.…”

“…85,234,399 Th ese joints might thus not be reliably compared in osteochondral defect healing models. In general, the eff ectiveness of DBM depends on numerous variables, including the age of the donor, 347 the tissue bank and the lot, 172,173,450 prolonged heat treatment, 196 the size or shape of the graft particles, 155 the nature of the carrier material, the extent of residual bone mineral content, 459 and the method of sterilization. 171 However, most of these variables are not applicable to the commercially available DBM used in the current study.…”

“…However, the use of human DBM in animals did not seem to aff ect positive results in other studies. 173,324 Hence, this aspect is probably not a good explanation for the lack of eff ectiveness. Th e immune response of the animals was not clearly related to the implantation of DBM; macrophages were observed in one DBM specimen and in four control specimens.…”

“…This preparation system produces 3 ml of PRP with a reported eightfold increase in platelet concentration and a fourfold to sevenfold increase in growth factor concentration compared with whole blood. 120,173,454 The concentration of platelets in the PRP of each subject in the present study was measured using an automated hematology analyzer (XE-5000, Sysmex, Japan) after 5 min of resuspension on a rocker, as recommended by Woodell-May et al 454 The median platelet concentration of the PRP was 1511 × 10 9 /l (range, 82 -2090 × 10 9 /l).…”

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

“…Compared to PPP and FCS, stimulation with PRP resulted in highest proteoglycan and collagen synthesis, whereas most of the collagen was identified as collagen type II. Spreafico et al (2009) Han et al (2009) showed that thrombin activation eliminates the chondroand osteoinductive potential of PRP. Two other studies (Doucet et al, 2005;Zaky et al, 2008) strengthen these results, reporting successful chondrogenic induction of BMSC; in fact, they did not apply thrombin but used several freeze-thaw cycles to activate PRP.…”