The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study

Navratilova, Zdenka; Losse, Stanislav; Petrová, Pavla; Sikorova, Katerina; Chabronova, Alzbeta; Petřek, Martin

doi:10.3390/life10090191

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…Tobacco-rich compounds such as aromatic amines and N-nitroso can induce DNA damage in the form of double-strand breaks, base modifications, or bulky adduct formation (Stern et al 2009 ). Recent studies have suggested the possibility of tobacco smoking-induced oncogenic or antioncogenic gene expression by miRNA regulation in BCa (Cumberbatch et al 2018 ; Navratilova et al 2020 ). Hence, the analysis of the effect of recurrent genetic aberration in smoking and non-smoking groups of patients is essential.…”

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confidence: 99%

Diagnostic and prognostic potential clustered miRNAs in bladder cancer

et al. 2022

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At specific genomic loci, miRNAs are in clusters and their association with copy number variations (CNVs) may exhibit abnormal expression in several cancers. Hence, the current study aims to understand the expression of miRNA clusters residing within CNVs and the regulation of their target genes in bladder cancer. To achieve this, we used extensive bioinformatics resources and performed an integrated analysis of recurrent CNVs, clustered miRNA expression, gene expression, and drug–gene interaction datasets. The study identified nine upregulated miRNA clusters that are residing on CNV gain regions and three miRNA clusters (hsa-mir-200c/mir-141, hsa-mir-216a/mir-217, and hsa-mir-15b/mir-16-2) are correlated with patient survival. These clustered miRNAs targeted 89 genes that were downregulated in bladder cancer. Moreover, network and gene enrichment analysis displayed 10 hub genes (CCND2, ETS1, FGF2, FN1, JAK2, JUN, KDR, NOTCH1, PTEN, and ZEB1) which have significant potential for diagnosis and prognosis of bladder cancer patients. Interestingly, hsa-mir-200c/mir-141 and hsa-mir-15b/mir-16-2 cluster candidates showed significant differences in their expression in stage-specific manner during cancer progression. Downregulation of NOTCH1 by hsa-mir-200c/mir-141 may also sensitize tumors to methotrexate thus suggesting potential chemotherapeutic options for bladder cancer subjects. To overcome some computational challenges and reduce the complexity in multistep big data analysis, we developed an automated pipeline called CmiRClustFinder v1.0 (https://github.com/msls-bioinfo/CmiRClustFinder_v1.0), which can perform integrated data analysis of 35 TCGA cancer types.

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Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic potential clustered miRNAs in bladder cancer

et al. 2022

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The role of miRNA-144-3p/Oprk1/KOR in nicotine dependence and nicotine withdrawal in male rats

Shao

Fei

Xiao

et al. 2023

Nicotine and Tobacco Research

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Introduction The kappa-opioid receptor (KOR) has been implicated in mediating the behavioral and biochemical effects associated with nicotine reward and withdrawal, however, its underlying mechanisms remain to be further explored. Methods Adult male Sprague-Dawley rats were used to established a nicotine dependence and withdrawal model by injecting nicotine (3 mg/kg/day, s.c.) or vehicle for 14 days, followed by the termination of nicotine for 7 days. Body weight gain, pain behaviors and withdrawal scores were assessed in succession. MicroRNA (miRNA) sequencing was performed, and quantitative real-time PCR (RT-qPCR) was used to detect the expression of candidate miRNAs and Oprk1. Western blotting was performed to examine KOR protein expression of KOR. Luciferase assay was conducted to validate the relationship of certain miRNAs/Oprk1. Results The behavioral results shown that nicotine dependence and withdrawal induced behavioral changes. Biochemical analyses demonstrated that miR-144-3p expression decreased and Oprk1/KOR expression increased in the prefrontal cortex, nucleus accumben, and hippocampus. Further investigation suggested that miR-144-3p exerted an inhibitory effect on Oprk1 expression in PC12 cells. Conclusions This study revealed that miR-144-3p/Oprk1/KOR might be a potential pathway underlying the adverse effects induced by nicotine dependence and withdrawal, and might provide a novel therapeutic target for smoking cessation. Implications This study demonstrate an impact of nicotine dependence and nicotine withdrawal on behavioral outcomes and the expressions of miR-144-3p/Oprk1/KOR in male rats. These findings have important translational implications given the continued use of nicotine and the difficulty in smoking cessation worldwide, which can be applied to alleviated the adverse effects induced by nicotine dependence and withdrawal, thus assist smokers to quit smoking.

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The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study

Cited by 2 publications

References 54 publications

Diagnostic and prognostic potential clustered miRNAs in bladder cancer

Diagnostic and prognostic potential clustered miRNAs in bladder cancer

The role of miRNA-144-3p/Oprk1/KOR in nicotine dependence and nicotine withdrawal in male rats

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