I , A continuous culture technique was used to study the phosphorus requirements of rumen micro-organisms. Solutions of artificial saliva containing 120, 80, 40 and 0 mg inorganic phosphorus (PJ/I were infused into the reaction vessels previously inoculated with rumen contents, resulting in Pi concentrations in the vessel contents of 48, 28, 4 and < 1 mg/l respectively. Various fermentative and synthetic characteristics were examined.2. In the vessel contents, concentrations of protozoa (about 0.9 x 105/ml) were not significantly affected by pi concentration. Total volatile fatty acids (VFA) produced averaged about 6.83 mmol/h with Pi levels of 48 and 28 mg/l. Reduction in Pi concentrations to 4 and < 1 mg/l resulted in significant reductions in total VFA to approximately 6.25 and 3.75 mmol/h respectively, accompanied by a rise in pH from 6.5 to 7.3. Ammonia-nitrogen values, which averaged about 131 mg/l at the higher I: concentrations, also increased with the lowest level of P,
5.It was estimated that the minimum pi concentrations required in rumen fluid in vivo to maintain maximum degradative and synthetic microbial activities was in the range 75-100 mg/l and that the over-all P requirement of the microbes was of the order of 5.1 g/kg apparently digested organic matter intake. Phosphorus deficiency is a major problem in many areas of the world. Most of the normal forages consumed by ruminants are little more than adequate with respect to their P content. Furthermore, increasing use of poor-quality roughages and by-products such as sugar-beet pulp, generally deficient in P, tends to exacerbate the problem. It has frequently been shown that feeding P-deficient diets could lead to decreased voluntary feed intake (Preston & Pfander, 1964;Coombe et al. 1971 ;Smith, 1984) with consequent effects on growth rate, milk production, etc. (Hemingway, 1967). These effects may be at least in part a result of impaired rumen function (Fishwick et al. 1977, Bass et al. 198 1 ;Durand et al. 1982; Breves & Holler, 1983). In general, ruminant feeds sustain the symbiotic rumen micro-organisms and only indirectly the host animal (Hungate, 1966) and it has been suggested that the P requirements of these organisms may be greater than that of the host animal (Preston & Pfander, 1964). There is, however, a strong interaction between the host animal and the rumen micro-organisms with respect to P supply and utilization, and in order to ascertain precise requirements of the micro-organisms, studies need to be carried out in situations which are not influenced by the host animal.In vitro work with pure cultures of rumen bacteria have shown the essentiality of P for growth (Bryant et al. 1959) and its effects on growth rates and yields (Caldwell et al. 1973;