Microalgae are potential renewable biodiesel raw materials, with the advantages of high oil yield per unit, no competition with farmland, and synergistic carbon dioxide fixation. However, microalgal biodiesel has not been widely commercialized so far, and the high cost of cultivation and recovery makes its product price less competitive with traditional petrochemical diesel. The main purpose of this paper is to study the design of experimental teaching of environmental microbiology based on the content of microalgae cultivation in the context of big data. Based on the analysis of the current situation of biology experiment teaching, this paper decides to adopt the method of design experiment teaching to carry out biology experiment teaching in high school. Experiments show that the impact of teaching on students' biological learning accounts for 31%, ranking after personal interests, which not only shows that teaching has a very important position in the factors that affect students' biological learning, but also shows that teaching is interesting, scientific, and diverse. Sex needs to be improved to further promote students' interest in biology.