The Effect of Valproate Therapy on Thrombin Generation Determined by Calibrated Automated Thrombography

Topf, Hans-Georg; Lischetzki, Grischa; Trollmann, Regina; Rascher, Wolfgang; Rauh, Manfred

doi:10.1055/s-0031-1275310

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…Routinely, venous blood was drawn 1–2 days prior to surgery as pre-operative check-up and directly post-operatively on our PICU, collected in tubes (Sarstedt, Nümbrecht, Germany) containing trisodium citrate as anticoagulant (ratio blood / anticoagulant = 9:1) or EDTA. Blood cell counts were measured using a blood cell analyser XE-2100 (Sysmex, Norderstedt, Germany), as previously described [ 18 ]. Platelet poor citrate plasma was obtained by centrifugation (10 min, 1 600*g) at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

“…Supernatant plasma was collected and centrifuged (10 min, 22,000 g, RT) in a micro-centrifuge to minimize residual platelets. Determination of Prothrombin time ratio (PT, % of reference) and FXIII activity (%) was performed in a BCS ® analyzer (Siemens, Marburg, Germany), using commercially available reagents [ 18 ]. FXIII activity can be indirectly monitored via ammonia release [ 19 ] that follows FXIII-catalyzed peptide cross-linkage to glycine ethyl esters [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

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Influence of factor XIII activity on post-operative transfusion in congenital cardiac surgery—A retrospective analysis

et al. 2018

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ObjectivesCoagulation factor XIII (FXIII) plays a key role in fibrin clot stabilization—an essential process for wound healing following cardiothoracic surgery. However, FXIII deficiency as a risk for post-operative bleeding in pediatric cardiac surgery involving cardiopulmonary bypass (CPB) for congenital heart disease (CHD) is controversially discussed. Thus, as primary outcome measures, we analyzed the association of pre-operative FXIII activity and post-operative chest tube drainage (CTD) loss with transfusion requirements post-operatively. Secondary outcomes included the influence of cyanosis and sex on transfusion.MethodsOur retrospective analysis (2009–2010) encompassed a single center series of 76 cardio-surgical cases with CPB (0–17 years, mean age 5.61 years) that were post-operatively admitted to our pediatric intensive care unit (PICU). The observational period was 48 hours after cardiac surgery. Blood cell counts and coagulation status, including FXIII activity were routinely performed pre- and post-operatively. The administered amount of blood products and volume expanders was recorded electronically, along with the amount of CTD loss. Uni- and multivariate logistic regression analysis was performed to calculate the associations (odds ratios) of variables with post-operative transfusion needs.ResultsFXIII activities remained stable following CPB surgery. There was no association of pre- and post-operative FXIII activities and transfusion of blood products or volume expanders in the first 48 hours after surgery. Similarly, FXIII showed no association with CTD loss. Cyanosis and female sex were associated with transfusion rates.ConclusionsAlthough essentially involved in wound healing and clotting after surgery, FXIII activity does not serve as a valid predictor of post-operative transfusion need.

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Section: Methodsmentioning

confidence: 99%