The Effect of Various pH Medium on the Secondary Metabollites Production from Trichoderma harzianum T10 to Control Damping Off on Cucumber Seedlings

Abstract

Damping-off is one of the main diseases in cucumber seedlings caused by Pythium sp. Secondary metabolites of Trichoderma harzianum T10 can conduct the control of the disease. The pH of the medium influences the production of secondary metabolites. The research aimed to determine the effective pH medium on production of T. harzianum T10 secondary metabolites, and the effect of the T. harzianum T10 secondary metabolites application in damping-off disease control also to the growth of cucumber seedling. The research was consist of two steps; 1) in vitro assay with various pH levels 5; 3; 3.5; 4; 4.5; 5.5; 6; 6.5; and 7, 2) In planta treatments consisted of control, fungicide (Mancozeb), secondary metabolites in pH 5 and 5.5 with the concentration of 5, 10 and 15% each. The research showed that: 1) the effective pH medium for the production of T. harzianum T10 secondary metabolites was 5 and 5.5. 2) application of the T. harzianum T10 secondary metabolites on pH 5 and 5.5 with a concentration of 5, 10, and 15% could decrease the disease incidence and support cucumber seedling growth.

Effect of Four Liquid Organic Wastes on The Growth of Four Trichoderma harzianum Isolates and Their Effect on Cucumber Growth and Yield

“…This is in accordance with the opinion Gayal et al (2017) that generally cucumber plants grow at the appropriate pH, namely pH 5.5-6.7. In addition, T. harzianum is also able to live at the pH in accordance with Chalimah et al (2020).…”