Human behavior data provides essential feedback information for architects to improve a human-centered indoor light environment design. However, architects have difficulty capturing the complex, multidimensional, and unpredictable behavior of humans, often struggle to get users’ feedback on time in the schematic phase. This paper proposes a new virtual reality-based behavioral measurement and assessment tool that quantitatively collects and analyzes individual behavioral data, including travel trajectory, travel time, and gaze points, to reveal user experience and interaction of light, aiming to better help architects get timely feedback from users and create human-centered indoor light environment designs in the scheme optimization phase. To showcase this tool, we utilize an exhibition hall of a museum design as an illustrative example. The experiment demonstrates the feasibility of the proposed tool, and its results suggest that different lighting schemes influence human behavior patterns and that the introduction of natural light usually stimulates more movement. The developed virtual reality tool prototype provides valuable visual information and statistics for analyzing human behavior and evaluating indoor light environment design schemes.