The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution

Rahman, Alief Putriana; Purwanto, Djoko; Isnaeni, Isnaeni

doi:10.20473/jfiki.v6i22019.62-68

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…The extract was cooled to room temperature then filtered. The GTE was prepared daily to prevent degradation of important constituents [25] and used in an oral dose of 300 mg/kg/day [26,27].…”

Section: Experimental Designmentioning

confidence: 99%

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

Khaleel¹,

Shaari²,

Nawi³

et al. 2021

Arch Med Sci Civil Dis

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Introduction: Ciprofloxacin is a broad-spectrum antibiotic which can cause a chondrotoxic effect in the growing condyle. Researchers found that green tea showed significant health benefits for a large number of disorders. The objective of the present research was to investigate the preventive potential of green tea extract against mandibular chondrotoxicity induced by ciprofloxacin in juvenile Wistar rats. Material and methods: Twenty juvenile male rats were used and divided into four equal groups, the saline/water (S/W), saline/green tea (S/G), ciprofloxacin/water (C/W) and ciprofloxacin/green tea (C/G) treated groups. On day 32 of age, all the animals in C/W and C/G treated groups were subcutaneously injected with ciprofloxacin as two subcutaneous injections of 600 mg/kg of body weight, eight hours apart, while the S/W and S/G groups were subcutaneously injected with saline. The S/G and C/G groups were intragastrically gavaged by green tea extract in an oral dose of 300 mg/kg/ day, 8 days before the subcutaneous injection of saline or ciprofloxacin. On day 34, all the animals were anaesthetized, and the mandibular condyle samples were taken immediately and processed. Results: In comparison with the C/W treated group, the C/G treated group showed a significant increase in the thickness of the mandibular condylar cartilage, cartilage cell number, glycosaminoglycan content, and Bcl-2 immune expression (p < 0.05). Conclusions: This study provides the first evidence that green tea extract can significantly decrease the chondrotoxic effects of ciprofloxacin in a rat mandibular condyle cartilage model.

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Section: Experimental Designmentioning

confidence: 99%

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

Khaleel¹,

Shaari²,

Nawi³

et al. 2021

Arch Med Sci Civil Dis

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The Effect of Manalagi Apple Juice (Malus Sylvestris Mill) on Stability and Antioxidant Activity of Black Tea

Purwanto¹,

Darmawati²

2022

bikfar

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Black tea (Camellia sinensis O.K var assamica) is one of the most widely consumed beverages by the public. Manalagi Apple (Malus sylvestris Mill) juice and black tea contain antioxidant compounds that can counteract free radicals. The purpose of this study was to prove that the addition of Manalagi apple juice would increase the antioxidant activity and stability of black tea. The DPPH (2,2-diphenyl-1-picrylhydrazyl) method was used in this study to determine their antioxidant activities. Black tea antioxidant activity was measured at 0, 6 and 24 hours after the addition of Manalagi apple juice. Antioxidant stability of black tea compared to black tea added with Manalagi apple juice. The results obtained showed that the antioxidant activity of a mixture of black tea and Manalagi apple juice was higher than the antioxidant activity of black tea alone. It was shown that the IC50 of a mixture of black tea and Manalagi apple juice was lower than black tea. The addition of Manalagi apple juice can also increase the stability of the antioxidants in the black tea solution and the slope of the linear equation % inhibition of the mixture is lower by about one third than black tea. Because of this reason, adding Manalagi apple juice will slow down the degradation rate of the antioxidant activity of black tea.

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The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution

Cited by 2 publications

References 10 publications

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

The Effect of Manalagi Apple Juice (Malus Sylvestris Mill) on Stability and Antioxidant Activity of Black Tea

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