The Effect of Vitamin D3 and Silver Nanoparticles on HaCaT Cell Viability

Cataldi, Samuela; Ceccarini, Maria Rachele; Patria, F.; Beccari, Tommaso; Mandarano, Martina; Ferri, Ivana; Lazzarini, Andrea; Curcio, Francesco; Albi, Elisabetta

doi:10.3390/ijms23031410

Cited by 14 publications

(5 citation statements)

References 40 publications

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“…MCF7 cells were cultured for 48 h, then centrifuged at 1000× g for 10 min and treated as previously reported [ 53 ]. The pellets were fixed in 10% neutral phosphate-buffered formaldehyde solution for 24 h. Then, the cytoinclusion technique by the Cellient ® Automated Cell Block System (Hologic, Mississauga, ON, Canada) that rapidly creates a paraffin-embedded cell block was used, as previously reported.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Cholesterol in MCF7 Human Breast Cancer Cells

Albi

Mandarano

Cataldi

et al. 2023

IJMS

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In the last decade, cholesterol level has been implicated in several types of cancer, including breast cancer. In the current study, we aimed to investigate the condition of lipid depletion, hypocholesterolemia or hypercholesterolemia reproduced in vitro to analyze the response of different human breast cancer cells. Thus, MCF7 as the luminal A model, MB453 as the HER2 model and MB231 as the triple-negative model were used. No effect on cell growth and viability was detected in MB453 and MB231 cells. In MCF7 cells, hypocholesterolemia (1) reduced cell growth, and Ki67 expression; (2) increased ER/PgR expression; (3) stimulated the 3-Hydroxy-3-Methylglutaryl-CoA reductase and neutral sphingomyelinase and; (4) stimulated the expression of CDKN1A gene coding cyclin-dependent kinase inhibitor 1A protein, GADD45A coding growth arrest and DNA-damage-inducible alpha protein and, PTEN gene coding phosphatase and tensin homolog. All these effects were exacerbated by the lipid-depleted condition and reversed by the hypercholesterolemic condition. The relationship between cholesterol level and sphingomyelin metabolism was demonstrated. In summary, our data suggest that cholesterol levels should be controlled in luminal A breast cancer.

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Section: Methodsmentioning

confidence: 99%

The Effect of Cholesterol in MCF7 Human Breast Cancer Cells

Albi

Mandarano

Cataldi

et al. 2023

IJMS

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“…MTT assay was used to test cell viability, as previously reported [35]. MCF7 and MB231 treated with increasing concentration of VitC from 0.1mM to 20.0mM were seeded into 96-well plates at a density of 1 × 10 4 cells/well with DMEM complete culture medium.…”

Section: Cell Viabilitymentioning

confidence: 99%

Vitamin C Modifies the Phenotype of Hormone Receptor Positive/HER2 Negative Breast Cancer Cells and Induces Changes in Sphingolipid Metabolism

Codini¹,

Fiorani²,

Mandarano³

et al. 2023

Preprint

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The effect of the antioxidant vitamin C in cancer has been studied for over 30 years with conflict-ing results ranging from total absence of effect, to possible protective effect, to potential therapeutic effect when administered in high doses. The aim of the study was to define the effect of vitamin C in breast cancer and the possible involvement of sphingomyelin metabolism. Therefore, the effect of low and high dose of vitamin C on cells reproducing luminal A type (MCF7) and triple negative type (MB231) of breast cancer was analyzed. The results showed that high dose of vitamin C produced effects only in MCF7 cells such as decreased cell viability, modulation of cell cycle re-lated genes, unexpected change in cell phenotype due to estrogen receptor downregulation, catabo-lism of sphingomyelin with a large increase in ceramide. In conclusion, we demonstrated that high doses of VitC on the one hand reduce the growth of lu-minal A type breast cancer cells and on the other hand induce their phenotypic change towards hormone therapy resistant cells. The effect of vitamin C on therapeutic treatment failure of luminal A breast cancer and the possible involvement of high ceramide levels were discussed.

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“…The MTT assay was used to test cell viability, as previously reported [50]. MCF7 and MDA-MB-231 were seeded in 96-well plates at a density of 1 × 10 4 cells/well with DMEM complete culture medium (see Section 4.2).…”

Section: Cell Viabilitymentioning

confidence: 99%

Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

Codini,

Fiorani,

Mandarano

et al. 2023

IJMS

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The role of sphingomyelin metabolism and vitamin C in cancer has been widely described with conflicting results ranging from a total absence of effect to possible preventive and/or protective effects. The aim of this study was to establish the possible involvement of sphingomyelin metabolism in the changes induced by vitamin C in breast cancer cells. The MCF7 cell line reproducing luminal A breast cancer and the MDA-MB-231 cell line reproducing triple-negative breast cancer were used. Cell phenotype was tested by estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2 expression, and proliferation index percentage. Sphingomyelin was localized by an EGFP-NT-Lys fluorescent probe. Sphingomyelin metabolism was analyzed by RT-PCR, Western blotting and UFLC-MS/MS. The results showed that a high dose of vitamin C produced reduced cell viability, modulated cell cycle related genes, and changed the cell phenotype with estrogen receptor downregulation in MCF7 cell. In these cells, the catabolism of sphingomyelin was promoted with a large increase in ceramide content. No changes in viability and molecular expression were observed in MB231 cells. In conclusion, a high dose of vitamin C induces changes in the luminal A cell line involving sphingomyelin metabolism.

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The Effect of Vitamin D3 and Silver Nanoparticles on HaCaT Cell Viability

Cited by 14 publications

References 40 publications

The Effect of Cholesterol in MCF7 Human Breast Cancer Cells

The Effect of Cholesterol in MCF7 Human Breast Cancer Cells

Vitamin C Modifies the Phenotype of Hormone Receptor Positive/HER2 Negative Breast Cancer Cells and Induces Changes in Sphingolipid Metabolism

Sphingomyelin Metabolism Modifies Luminal A Breast Cancer Cell Line under a High Dose of Vitamin C

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