The effect of warming on mortality, metabolic rate, heat-shock protein response and gonad growth in thermally acclimated sea urchins (Heliocidaris erythrogramma)

Harianto, Januar; Nguyen, Hong D.; Holmes, Sebastian P.; Byrne, Maria

doi:10.1007/s00227-018-3353-8

Cited by 44 publications

(40 citation statements)

References 86 publications

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“…We found just two records of greater temperature ranges in similar intertidal habitats in California (8.2–45.0°C; Kelly et al, 2011) and the Mediterranean (7.0–35.0°C; Sarà et al, 2014). These fluxes reflect the extreme conditions that resident species are currently adapted to, many of which at our study site have broad physiological thermal tolerance (Brothers et al, 2016; Carey et al, 2016; Christensen et al, 2011; Davis et al, 2013; Hardy et al, 2014; Harianto et al, 2018; McElroy et al, 2012; Nguyen & Byrne, 2014; Pecorino et al, 2014; Wolfe et al, 2013a, 2013b). These ‘thermal generalists’, which are typical of temperate ecosystems, are predicted to fare comparatively well in a warming ocean (Huey & Hertz, 1984; Madeira, Narciso, Cabral, & Vinagre, 2012; Tewksbury, Huey, & Deutsch, 2008), but there may be a limit.…”

Section: Discussionmentioning

confidence: 76%

“…and pH −0.5 suggests that this species may be living close to their thermal tolerance limits in the region (Carey et al, 2016;Harianto et al, 2018), as evidenced by the link between warming and increased mortality and disease (Brothers et al, 2016;Sweet, Bulling, & Williamson, 2016). Formation of a weaker more porous skeleton under acidification conditions in H. erythrogramma (Byrne & Fitzer, 2019;Johnson et al, 2020;Wolfe et al, 2013a) reflects the reduced calcification and smaller size of sea urchins and other calcifiers when exposed to low pH (Byrne et al, 2010;Dworjanyn & Byrne, 2018;Byrne & Fitzer, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Biogeochemical dynamics were determined to achieve a detailed assessment of the environmental conditions and patterns of ecological stress (sensu Alexander, 1999; herein: ‘eco‐stress’) experienced by resident biota. The pools were chosen because they support a diverse community of species for which tolerance levels to acidification and warming, as individual or combined stressors, have been determined ( echinoderms: Brothers, Harianto, McClintock, & Byrne, 2016; Carey, Harianto, & Byrne, 2016; Christensen, Nguyen, & Byrne, 2011; Hardy et al, 2014; Harianto, Nguyen, Holmes, & Byrne, 2018; Johnson, Harianto, Thomson, & Byrne, 2020; McElroy, Nguyen, & Byrne, 2012; Nguyen & Byrne, 2014; Nguyen et al, 2012; Pecorino, Barker, Dworjanyn, Byrne, & Lamare, 2014; Wolfe, Dworjanyn, & Byrne, 2013a; Wolfe, Dworjanyn, & Byrne, 2013b; molluscs: Coleman, Byrne, & Davis, 2014; Davis et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Characterizing biogeochemical fluctuations in a world of extremes: A synthesis for temperate intertidal habitats in the face of global change

Wolfe

Nguyen

Davey

et al. 2020

Global Change Biology

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Coastal and intertidal habitats are at the forefront of anthropogenic influence and environmental change. The species occupying these habitats are adapted to a world of extremes, which may render them robust to the changing climate or more vulnerable if they are at their physiological limits. We characterized the diurnal, seasonal and interannual patterns of flux in biogeochemistry across an intertidal gradient on a temperate sandstone platform in eastern Australia over 6 years (2009–2015) and present a synthesis of our current understanding of this habitat in context with global change. We used rock pools as natural mesocosms to determine biogeochemistry dynamics and patterns of eco‐stress experienced by resident biota. In situ measurements and discrete water samples were collected night and day during neap low tide events to capture diurnal biogeochemistry cycles. Calculation of pHT using total alkalinity (TA) and dissolved inorganic carbon (DIC) revealed that the mid‐intertidal habitat exhibited the greatest flux over the years (pHT 7.52–8.87), and over a single tidal cycle (1.11 pHT units), while the low‐intertidal (pHT 7.82–8.30) and subtidal (pHT 7.87–8.30) were less variable. Temperature flux was also greatest in the mid‐intertidal (8.0–34.5°C) and over a single tidal event (14°C range), as typical of temperate rocky shores. Mean TA and DIC increased at night and decreased during the day, with the most extreme conditions measured in the mid‐intertidal owing to prolonged emersion periods. Temporal sampling revealed that net ecosystem calcification and production were highest during the day and lowest at night, particularly in the mid‐intertidal. Characterization of biogeochemical fluctuations in a world of extremes demonstrates the variable conditions that intertidal biota routinely experience and highlight potential microhabitat‐specific vulnerabilities and climate change refugia.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterizing biogeochemical fluctuations in a world of extremes: A synthesis for temperate intertidal habitats in the face of global change

Wolfe

Nguyen

Davey

et al. 2020

Global Change Biology

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“…In order to assess such an issue, we made efforts in the evaluation of the protein level of HSP60, HSP70 and HSP90. The HSPs exert a protective role as chaperones, by assisting protein folding and preventing their aggregation also in response to stressors including ROS, warming and metals [ 67 , 68 , 69 , 70 , 71 ]. Accordingly, their upregulation confirms the activation of a defence mechanism in the P. lividus gonads, which act to cope with the stress so as to tolerate, at least, the rate of ROS.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Changes after Parental Exposure to Metals in the Sea Urchin Affect Timing of Genetic Programme of Embryo Development

Masullo

Biondo

Natale

et al. 2021

Biology

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It is widely accepted that phenotypic traits can be modulated at the epigenetic level so that some conditions can affect the progeny of exposed individuals. To assess if the exposure of adult animals could result in effects on the offspring, the Mediterranean sea urchin and its well-characterized gene regulatory networks (GRNs) was chosen as a model. Adult animals were exposed to known concentrations of zinc and cadmium (both individually and in combination) for 10 days, and the resulting embryos were followed during the development. The oxidative stress occurring in parental gonads, embryo phenotypes and mortality, and the expression level of a set of selected genes, including members of the skeletogenic and endodermal GRNs, were evaluated. Increased oxidative stress at F0, high rates of developmental aberration with impaired gastrulation, in association to deregulation of genes involved in skeletogenesis (dri, hex, sm50, p16, p19, msp130), endodermal specification (foxa, hox11/13b, wnt8) and epigenetic regulation (kat2A, hdac1, ehmt2, phf8 and UBE2a) occurred either at 24 or 48 hpf. Results strongly indicate that exposure to environmental pollutants can affect not only directly challenged animals but also their progeny (at least F1), influencing optimal timing of genetic programme of embryo development, resulting in an overall impairment of developmental success.

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“…Studies relating to sea urchin gonad proteins have mainly focused on physiological aspects, including environmental effects on Heliocidaris erythrogramma growth and identification of the gene expression of S. intermedius (Harianto, Nguyen, Holmes, & Byrne, 2018; Wang et al., 2018). Major yolk protein (MYP) is the main protein in sea urchin gonad due to the highest content (Wang, Ura, & Takagi, 2015), and localized to the egg and fluid of adult sea urchin as well (Dev & Robinson, 2014).…”

Section: Introductionmentioning

confidence: 99%

Functional properties of gonad protein isolates from three species of sea urchin: a comparative study

Shang

Yan

et al. 2020

Journal of Food Science

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Sea urchin Mesocentrotus nudus, Glyptocidaris crenularis, and Strongylocentrotus intermedius gonad protein isolates (mnGPIs, gcGPIs, and siGPIs) were extracted by isoelectric solubilization/precipitation (ISP) from the defatted gonads, and their functional properties were compared. Sodium dodecyl sulfate polyacrylamide gel electrophoresis results showed the similar protein pattern between each protein isolate and defatted gonad, indicating the high efficiency of ISP processing for protein recovery. Amino acid profileconfirmed that the mnGPIs and siGPIs could be potential sources of essential amino acid in nature. As regard to functional properties, mnGPIs showed higher water‐ and oil‐ holding capacities followed bysiGPIs and gcGPIs and all protein isolates presented great foaming property. As for emulsifying activity index (EAI), mnGPIs, gcGPIs, and siGPIs showed the minimum solubility and EAI at pH 5, 3, and 4, respectively, and behaved a pH‐dependent manner. The gcGPIs revealed the highest EAI from pH 6 to 8 among the samples. In addition, circular dichroism showed increased content of β‐sheet at the expense of α‐helix and β‐turn, suggesting the structure denaturation of the protein isolates. Indeed, no statistical difference was observed between secondary structure of mnGPIs and siGPIs. Moreover, ISP processing increased free sulfhydryl content of sea urchin protein isolates, but no difference was observed among the samples. Furthermore, siGPIs revealed the highest amount of total sulfhydryl and disulfide bonds, whereas both defatted gonads and protein isolates from G. crenularis presented the maximum surface hydrophobicity. These results suggest that gonad protein isolates from three species of sea urchin possess various functionalities and therefore can be potentially applied in food system. Practical Application Sea urchin M. nudus, G. crenularis, and S. intermedius gonads are edible, whereas the functional properties of protein isolates from sea urchin gonad remain unknown. In this case, the extraction and comparison of three species of sea urchin gonad protein isolates will not only confirm functional properties but also screen food ingredients with suitable functions. In this study, functionalities of protein isolates derived from M. nudus, G. crenularis, and S. intermedius gonads would provide potential application in bakery food and meat products or as emulsifier candidates in food system.

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The effect of warming on mortality, metabolic rate, heat-shock protein response and gonad growth in thermally acclimated sea urchins (Heliocidaris erythrogramma)

Cited by 44 publications

References 86 publications

Characterizing biogeochemical fluctuations in a world of extremes: A synthesis for temperate intertidal habitats in the face of global change

Characterizing biogeochemical fluctuations in a world of extremes: A synthesis for temperate intertidal habitats in the face of global change

Gene Expression Changes after Parental Exposure to Metals in the Sea Urchin Affect Timing of Genetic Programme of Embryo Development

Functional properties of gonad protein isolates from three species of sea urchin: a comparative study

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