The Effect of Whole Blood and Bone Marrow with the Addition of Pyrimidine-2,4(1h,3h)-dione Thietanyl Derivatives on Free Radical Oxidation

Abstract

Background: It is relevant to study the general patterns and to identify non-specific mechanisms of body protective and adaptive reactions violation, which can lead to the various pathological processes and to develop principles for the correction of these disorders. One of the therapy and prevention directions is the search for new medicines. In recent years, new derivatives of pyrimidine bases have been synthesized and studied. Pyrimidine based medicines have a membrane-stabilizing, immunomodulatory effect, normalize metabolic disorders, and increase the oxidative activity of leukocytes. Disruption of the free radical oxidation processes – the generation of reactive oxygen species and lipid peroxidation, including in whole blood and bone marrow, has gained importance in recent years. Methods: Each reaction was monitored by thin layer chromatography. 1H, 13C, 15N NMR spectra were recorded (chemical shifts were expressed as δ-values). We studied the effect of 6-methyl-3-(thietan-3-yl)pyrimidine-2,4(1H,3H)-dione on the generation of reactive oxygen species (ROS) in the whole blood and bone marrow using study of whole blood spontaneous and stimulated chemiluminescence (CL). CL methods make it possible to quickly and easily assess the studied material (whole blood, bone marrow) effect on free radical oxidation. Using CL methods it is possible to reveal the presence of medicines pro- or antioxidant properties, opening up new possibilities in the search for substances with antioxidant properties and comparison their activity. Results: Alkylation of 6-methylpyrimidine-2,4(1H,3H)-dione by 2-chloromethylthiirane in protic solvents in the presence of alkali leads to the formation of an N-thietane derivative. NMR spectroscopy showed that 6-methylpyrimidine-2,4(1H,3H)-dione is alkylated at position 3 The oxidation reactions of N-(thietan-3-yl)pyrimidine-2,4(1H,3H)-dione were studied and it was determined that, depending on the excess of the oxidizing agent and the duration of the process, N-(1-oxothietan-3-yl)- or N-(1,1-dioxothietan-3-yl)pyrimidine-2,4(1H,3H)-diones are formed. The effect of free radical oxidation processes of new biologically active pyrimidine-2,4(1H,3H)-diones were studied. Conclusion: New pyrimidine-2,4(1H,3H)-diones increase the general adaptive capabilities of the body and have protective effects in extreme conditions.

Siringaldehit Bazlı Yeni 2,4,6-Triarilpiridin Türevlerinin Antioksidan Aktiviteleri ve Teorik Profili

“…As a result, the search for antioxidant compounds has expanded to various fields of research, including nutrition, pharmacology, and biotechnology. In nutrition, researchers are investigating the antioxidant capacity of different foods and dietary supplements, with the goal of identifying dietary patterns and individual nutrients that can help prevent chronic diseases [14]. In pharmacology, researchers are exploring the potential of antioxidant compounds as therapeutic agents for various diseases.…”