The effect of X-irradiation on cell cycle progression and chromatid aberrations in stimulated human lymphocytes using cohort analysis studies

Aghamohammadi, S.Z.; Savage, John R. K.

doi:10.1016/0027-5107(92)90228-t

Cited by 19 publications

(6 citation statements)

References 21 publications

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“…The degree of delay seemed to depend upon the cell cycle position of the cells at the time of irradiation. Cells exposed earlier in the cell cycle appeared to be less delayed than those irradiated in the G 2 , which agrees with other studies (Aghamohammadi and Savage, 1992;Elkind and Whitmore, 1967). In mammalian cells ionising irradiation causes a mitotic delay, which may have several components including a G 1 block, an S phase delay, or a G 2 arrest (Maity et al, 1994).…”

Section: Radiation-induced Division Delaysupporting

confidence: 92%

Cell cycle dependent aneuploidy induction by x-rays in vitro in human lymphocytes

Tallon¹,

Verschaeve²,

Kirsch‐Volders³

1998

Microsc. Res. Tech.

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Section: Radiation-induced Division Delaysupporting

confidence: 92%

Cell cycle dependent aneuploidy induction by x-rays in vitro in human lymphocytes

Tallon¹,

Verschaeve²,

Kirsch‐Volders³

1998

Microsc. Res. Tech.

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“…Thus, it can be assumed that when Al was added for the first 4 h of culture time, cells in the G 0 / G 1 phase were treated. When Al was added after 10 hours of culture time and was kept until harvest, predominantly S and G 2 cells were exposed although, given the variable transit times of lymphocytes through the cell cycle (Aghamohammadi and Savage, 1992), treatment of some slow lymphocytes, those still in G 1 , cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

et al. 2005

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Although aluminum (Al) is responsible for the etiology of some human diseases, not much is known about the mechanisms of its genotoxic activity. The available data suggest that Al can induce DNA damage by modifying the structure of chromatin through the induction of reactive oxygen species or by damaging lysosomal membranes and liberating DNase. We treated human peripheral-blood lymphocytes with AlCl3 in the G0/G1 phase, in the S/G2 phase, and during the whole cell cycle. The aim of the study was to check if the sensitivity of lymphocytes to Al varied through the cell cycle. A high sensitivity in the S phase would point toward chromatin modification as the major source of DNA damage. Micronuclei (Mn) and apoptosis were assessed as the end points. Cells were treated with 1, 2, 5, 10, and 25 microg/mL AlCl3. Mn induced by 5 microg/mL of AlCl3 were analyzed by FISH for centromeric signal content. After all treatment schemes the frequency of Mn increased initially, but decreased at high AlCl3 concentrations. This drop of Mn frequency could be explained by a strong increase in the frequency of apoptosis. AlCl3 induced both Mn with and without centromeres. The G0/G1 phase of the cell cycle was found to be more sensitive than were the S and G2 phases. This points toward oxidative stress or liberation of DNase as the major source of DNA damage induced by Al.

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“…What is needed is some G 2 cell marker system that will allow us to unscramble scored cells and replace them in the correct developmental sequence. This can be done for S-phase cells utilizing the very precise band replication sequences (Savage and Bhunya, 1980;Aghamohammadi and Savage, 1992). Until we have such a system for G 2 , the progressive effects on aberrations of condensation and spatial alterations during transit, and the true shapes of dose-response curves, will remain matters for conjecture.…”

Section: Discussionmentioning

confidence: 99%

On the nature of visible chromosomal gaps and breaks

Savage¹

2004

Cytogenet Genome Res

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From the earliest days of chromosomal aberration studies, the distinction, nature and origin of light-microscope observed “gaps” and “breaks” have been topics for debate and controversy. In this paper we survey, briefly, the various ideas that have appeared in the very extensive literature, and attempt to evaluate them in the light of our current understanding of chromosome structure and aberration formation. Attention is drawn to the problems of interpretation caused by G₂/S cell imprecision.

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The effect of X-irradiation on cell cycle progression and chromatid aberrations in stimulated human lymphocytes using cohort analysis studies

Cited by 19 publications

References 21 publications

Cell cycle dependent aneuploidy induction by x-rays in vitro in human lymphocytes

Cell cycle dependent aneuploidy induction by x-rays in vitro in human lymphocytes

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

On the nature of visible chromosomal gaps and breaks

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