The effect of ω-fatty acids on the expression of phospholipase A ₂ group 2A in human gastric cancer patients

Abstract

Background:Studies show that polyunsaturated fatty acids (PUFAs) may have an inhibitory role in carcinogenesis. It was previously shown that PLA2 group 2A (PLA2G2A) messenger RNA (mRNA) expression is associated with less frequent metastasis and longer survival in gastric adenocarcinoma. This study intends to investigate the effect of PUFAs on the expression of PLA2G2A in patients with gastric cancer.Materials and Methods:Thirty-four patients with gastric cancer (GC) were randomly divided into two groups. The first group received cisplatin medication. The second group received cisplatin medication and supplements of ω-fatty acids for three courses. The total RNA was extracted from the tissues and cDNA was synthesized. The gene expression of PLA2G2A was evaluated by the real-time polymerase chain reaction (PCR) method. To confirm the changes in gene expression, frozen section was utilized. The frozen tissue samples were sectioned and stained using the immunohistochemistry technique.Results:After chemotherapy and chemotherapy plus supplement, the relative mean of PLA2G2A gene expression increased 1.5 ± 0.5-fold and 7.4 ± 2.6-fold, respectively (P = 0.006). The relative mean of gene expression in patients who received cisplatin and ω-fatty acids supplement increased more significantly (7.5 ± 3.3-fold) than in patients who received only cisplatin (P = 0.016).Conclusion:It was found that PUFAs increased the gene and protein expression of PLA2G2A in gastric cancer. Concerning the fact that studies reveal protective function of PLA2G2A in gastric cancer, it is suggested that increased expression of PLA2G2A is helpful. Furthermore, PUFAs can be considered as a useful therapeutic supplement for patients with gastric cancer.

Stachydrine represses the proliferation and enhances cell cycle arrest and apoptosis of breast cancer cells via PLA2G2A/DCN axis

“…PLA2G2A have the strongest bactericidal activities and may be implicated in cell signaling, apoptosis, cell membrane remodeling, and inflammatory responses (Dong et al., 2017). In gastric cancer, PLA2G2A expression is negatively correlated with infiltration depth, lymph node metastasis, and tumor‐lymph node‐metastasis stage (Shariati et al., 2016). Besides, targeting arachidonic acid (AA) pathway (a metabolic process playing key roles in carcinogenesis) and its metabolic enzymes including Phospholipase A 2 s (PLA 2 s) by natural products can be a possible way for cancer prevention and therapy (Yarla et al., 2016).…”