The Effect on the Transcriptome of Anemone coronaria following Infection with Rust (Tranzschelia discolor)

Abstract

In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3’end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

Morphological and transcript changes in the biosynthesis of lignin in oil palm (Elaeis guineensis) during Ganoderma boninense infections in vitro

“…C). Similar expression patterns for the COMT gene were displayed in Blumeria graminis ( Bgt )‐infected wheat (Bhuiyan et al ) and rust‐infected Anemone coronaria (Laura et al ). Initial downregulation of EgCOMT in T3 plantlets was most likely because of the suppression of plant defense as a result of upregulation of the putative fungal secreted proteins during the initial phase of infection.…”

Phytochemical and Antinociceptive Investigations of Anemone coronaria Active Part Ameliorating Diabetic Neuropathy Pain

“…However, the highest dose of AC showed more amelioration of tactile allodynia than those of SARF and compound 3. Thus, AC showed more powerful antinociceptive activity against thermal hyperalgesia and mechanical allodynia than its most active fraction (SARF) and constituent glaucine (3), showing that there is an apparent additive effect between AC constituents potentiating its activity as concluded before in literature [12,20,29,30].…”

“…Anemone coronaria L. belongs to the family Ranunculaceae and is a winter perennial, flowering in the Mediterranean region from February until May [2]. A. coronaria was reported to contain anthocyanins (flowers), triterpene glycosides (tubers), and alkaloids (herb) [3][4][5][6]. A. coronaria has been used as antineuralgic and antirheumatic in the folk medicine of Lebanon [7].…”

Sequencing and de novo transcriptome assembly of Anthopleura dowii Verrill (1869), from Mexico

“…Nonetheless, only a limited number of transcriptomes from sea anemones have been sequenced so far [6], [7], [8], [9], [10], [11] Here we performed de novo transcriptome assembly for the sea anemone Anthopleura dowii Verrill (1869) (Actiniaria: Enthemonae: Actinioidea: Actiniidae) by next-generation sequencing.…”