The effect varied scanning electron microscopy desiccation techniques has on demineralized dentin

Abstract

The study objective was to assess (a) the effect of a rubbing‐application of ethylenediaminetetraacetic acid (EDTA) or citric acid (CA) has on the ultrastructure of surface dentin and (b) the effect of two scanning electron microscopy (SEM) desiccation preparation techniques have on the collagen surface produced. Treatment regions on proximal root surfaces of extracted human teeth were root planned to expose dentin. Cotton pellets soaked in either 30% CA or 24% EDTA solution were rubbed on the treatment region then processed for SEM using one of two desiccation techniques, that is, (a) critically point dried from liquid CO2 (control) or (b) air‐dried from tetramethylsilane (experimental). Specimens were coated with gold/palladium and viewed/photographed with an SEM. Specimens of the control groups displayed tufted fibrils (CA > EDTA) with many dentin tubules being partially obscured by overhanging fibrils. Air‐dried specimens of both treatment groups displayed a flat intact monolayer devoid of a matted meshwork of fibrous collagen. Discrete fibril “sprigs,” emanating from the surface monolayer, were characteristic of the EDTA group only. The rubbing‐application of EDTA on dentin produces a tufted fibril surface somewhat similar to that produced by CA. Air‐drying desiccation of both resulted in marked distortion with fibril collapse/coalescence of the tufted collagen matrix.

A Drop‐By‐Drop Self‐Assembled All‐Natural Hydrogel as a Desensitizer for Rapid and Enduring Management of Dentin Hypersensitivity

“…To mimic the impact of friction and etching in an oral environment, dentin slices were subjected to a 2 wt% EDTA aqueous solution for 2 min following mechanical friction application. [ 39 ] Specifically, the initial airtightness test yielded 15 data points for each group labeled “before” in Figure 2H. Subsequent to mechanical friction on the same samples, the second airtightness test was conducted, providing 15 data points for each group labeled “after brushing” in Figure 2H.…”