The effective constituent puerarin, from
            <i>Pueraria lobata</i>
            , inhibits the proliferation and inflammation of vascular smooth muscle in atherosclerosis through the miR-29b-3p/IGF1 pathway

Li, Jianpeng; Li, Yanan; Yuan, Xia; Yao, Dengfeng; Gao, Zongyue; Niu, Zhaoyang; Wang, Zheng; Zhang, Yue

doi:10.1080/13880209.2022.2099430

Cited by 17 publications

(7 citation statements)

References 37 publications

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“… 33 , 34 In addition, a recent study on atherosclerosis showed that IGF1 pathway could regulate the inflammation and proliferation of vascular smooth muscle cells. 35 In AD-related disease, IGF1 has been reported to be positively correlated with an increased aortic diameters 36 in Turner syndrome, which is a common cause of AD. NOS3 is a key player in governing angiogenesis, and its aberrant expression will result in the dysfunction of vascular endothelium.…”

Section: Discussionmentioning

confidence: 99%

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Zheng,

Yang,

Liu

et al. 2024

JIR

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Purpose Immune microenvironment plays an important role in aortic dissection (AD). Therefore, novel immune biomarkers may facilitate AD prevention, diagnosis, and treatment. This study aimed at mining key immune-related genes and relevant mechanisms involved in AD pathogenesis. Patients and Methods Key immune cells in AD were identified by ssGESA algorithm. Next, genes associated with key immune cells were screened by weighted gene coexpression network analysis (WGCNA). Then hub immune genes were picked from protein-protein interaction network of overlapped genes from differential expression and WGCNA analyses by cytohubba plug-in. Their diagnostic potential was evaluated in two independent cohorts from GEO database. In addition, the expressions of hub immune genes were determined by quantitative RT-PCR, immunohistochemistry, and Western blotting in dissected and normal aortic tissues. Results Activated B cells, CD56dim natural killer cells, eosinophils, gamma delta T cells, immature B cells, natural killer cells and type 17 T helper cells were identified as key immune cells in AD. Thereafter, a gene module significantly correlated with key immune cells were found by WGCNA method. Subsequently, KDR, IGF1, NOS3, PECAM1, GAPDH, FLT1, DLL4, CDH5, VWF, and TEK were identified as hub immune cell related genes by PPI network analysis, which may be potential diagnostic markers for AD, as evidenced by ROC curves. Moreover, the decreased expression of VWF in AD was validated at both mRNA and protein levels, and its expression was significantly positive correlated with the marker of smooth muscle cells, ACTA2, in AD. Further immunofluorescent results showed that VWF was colocalized with ACTA2 in aortic tissues. Conclusion We identified key immune cells and hub immune cell-related genes involved in AD. Moreover, we found that VWF was co-expressed with the smooth muscle cell marker ACTA2, indicating the important role of VWF in smooth muscle cell loss in AD pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Zheng,

Yang,

Liu

et al. 2024

JIR

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“…Li et al . [ 33 ] found that puerarin can inhibit the secretion of interleukin-6 and interleukin-8 by atherosclerotic vascular smooth muscle, mainly through the miR-29b-3p/IGF1 axis to inhibit inflammation. Tofacitinib inhibits the production of pro-inflammatory factors in rheumatoid arthritis synovial fibroblasts, in which IGF1 plays an important role [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion

Ma,

Li,

Chen

et al. 2023

Adipocyte

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Background Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa. Methods OvCa cells were maintained in the adipocyte-conditioned medium. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, separately. Proliferation and apoptosis-related protein expression were assayed by western blot. The IC 50 values of cisplatin and carboplatin were determined using CCK-8. IGF1 secretion and expression were assayed via ELISA and western blot, respectively. A xenograft model was established, and pathological changes were detected by H&E staining. Proliferation and apoptosis-associated protein expression was assessed via IHC. Results Adipocytes promoted the viability and repressed cell apoptosis in OvCa, as well as enhancing platinum resistance, while the addition of IGF-1 R inhibitor reversed the effects of adipocytes on proliferation, apoptosis, and drug resistance of OvCa cells. Treatment with different concentrations of Ojeok-san (OJS) inhibited the adipocyte-induced platinum resistance in OvCa cells by suppressing IGF1. The combined treatment of OJS and cisplatin significantly inhibited tumour growth in vivo with good mouse tolerance. Conclusion In summary, OJS inhibited OvCa proliferation and platinum resistance by suppressing adipocyte paracrine IGF1 secretion.

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“…Puerarin, an active monomer in Pueraria lobata, was reported to inhibit the proliferation and inflammation of VSMCs in atherosclerosis by reducing the expression of miR-29b-3p, thereby increasing the expression of insulin-like growth factor 1 (IGF1) (J. Li et al, 2023). Therefore, puerarin may have a beneficial effect in the treatment of atherosclerosis by regulating miRNA.…”

Section: Puerarinmentioning

confidence: 99%

“…In vitro: hVSMCs (J. Li et al, 2023) MKP-1 mitogen-activated protein kinase phosphatase 1, PTEN phosphatase and tensin homolog, ABCA1 ATP-binding cassette transporter A1, PCSK9 proprotein convertase subtilisin/kexin type 9, VLDLR very low-density lipoprotein receptor, KLF4 krüppel-like factor 4, DUSP5 dual specificity phosphatase 5, MGP matrix gla protein, MyD88 myeloid differentiation primary response 88, TLR4 toll-like receptor 4, SIRT1 sirtuin-1, p53 tumor protein 53, WNT5A wingless/integrated-5A, TPM1 tropomyosin 1, DDAH1 dimethylarginine dimethylaminohydrolase 1, HMGB1 high mobility group box 1, EZH2 enhancer of zeste homolog 2, Smad3 sma-and mad-related protein 3, STAT3 signal transducer and activator of transcription 3, IGF1 insulin-like growth factor 1, ApoE -/mice apolipoprotein e-knockout mice, RAW264.7 RAW 264.7 mouse leukemia macrophage cell line, HUVECs human umbilical vein endothelial cells, THP-1 human acute monocytic leukemia cell line, VSMCs vascular smooth muscle cells, HA-VSMCs human aortic vascular smooth muscle cells, HASMCs human airway smooth muscle cells, HAEC human aortic endothelial cells, SD rats sprague-dawley rats, RAECs rat aortic endothelial cells, VECs vascular endothelial cells, hVSMCs human vascular smooth muscle cells, TET2 tet methylcytosine dioxygenase 2.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

与非编码调节相关的抗动脉粥样硬化机制中药活性单体的rna

Liu

Tan

Zhao

et al. 2023

Preprint

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Atherosclerosis is the leading cause of numerous cardiovascular diseases with a high mortality rate. Non-coding RNAs (ncRNAs), RNA molecules that do not encode proteins in human genome transcripts, are known to play crucial roles in various physiological and pathological processes. Recently, researches on the regulation of atherosclerosis by ncRNAs, mainly including microRNAs, long non-coding RNAs, and circular RNAs, have gradually become a hot topic. Traditional Chinese medicine has been proved to be effective in treating cardiovascular diseases in China for a long time, and its active monomers have been found to target a variety of atherosclerosis-related ncRNAs. These active monomers of traditional Chinese medicine hold great potential as drugs for the treatment of atherosclerosis. Here, we summarized current advancement of the molecular pathways by which ncRNAs regulates atherosclerosis and mainly highlighted the mechanisms of traditional Chinese medicine monomers in regulating atherosclerosis through targeting ncRNAs.

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The effective constituent puerarin, from Pueraria lobata , inhibits the proliferation and inflammation of vascular smooth muscle in atherosclerosis through the miR-29b-3p/IGF1 pathway

Cited by 17 publications

References 37 publications

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion

与非编码调节相关的抗动脉粥样硬化机制中药活性单体的rna

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The effective constituent puerarin, from Pueraria lobata , inhibits the proliferation and inflammation of vascular smooth muscle in atherosclerosis through the miR-29b-3p/IGF1 pathway

Cited by 17 publications

References 37 publications

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Identification of Key Immune Infiltration Related Genes Involved in Aortic Dissection Using Bioinformatic Analyses and Experimental Verification

Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion

与非编码调节相关的抗动脉粥样硬化机制 中药活性单体的rna

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Product

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与非编码调节相关的抗动脉粥样硬化机制中药活性单体的rna