The Effective Mechanism of Heat Stress on Mouse Embryo Implantation

Abstract

By imitating to establish a pattern, which is high temperature environment in summer,effect the stress of pregnant mice, discussed preliminarily the mechanism that heat stress influence on SPF kunming mice embryo implantation. The results of this study shows that the pregnant mice at the embryos preimplantation is stressed 42°C for 2h, which could down-regulated E2 and P4 levels of blood serum and protein expression,and then down-regulated the LIF, VEGF mRNA and their proteins expressions on pregnancy 5d (after implantation),and up-regulated COX-2 mRNA and its protein expression, but down-regulated HOXa-10 mRNA expression;VEGF, COX-2 mRNA and their proteins expression were up-regulated on pregnancy 6d,eventually caused the embryos implantation and litter-size decreased.