The Effectiveness Of Anchovy Concentration  (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

Fa’Izah, Almira; Wardani, Istien; Soesilo, Diana

doi:10.30649/denta.v10i1.37

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…The use of fluoride has an inhibitory effect on the growth of the cause of dental caries. The mechanism of fluorine in preventing and reducing caries is by inhibiting glycolysis and reducing acid production from cariescausing bacteria, increasing the mineralization process and reducing demineralization 16 .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Power of Nano Brown Anchovy (<i>Stolephorus insularis</i>) Against Mixed Bacteria in Deep Dentin Caries

Yuanita

Paramitha

Mustamu

et al. 2023

IJMDS

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Background: Biodentin is a pulp capping material that has perfected the shortcomings of Ca(OH)2, but has low radiopacity and lower washing out resistance, so it is hoped that there will be natural ingredients that can be used as pulp capping materials. The nano brown anchovy has antibacterial content in the form of fluoride. Its nano size can also facilitate penetration better. Purpose: Analyzing the antibacterial potency of nano brown anchovy on mixed bacteria in deep carious dentine. Methods: The research was conducted in an experimental laboratory in vitro with a post-test only control group design. Brown anchovy was converted into nanoparticles then diluted into several concentrations using the broth dilution method. Direct contact method was used between nano anchovy and various concentrations of bacteria. The values of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bacterial Concentration) were obtained by counting the number of bacterial colonies growing on Mueller Hinton Agar media. Colony growth was calculated manually in Colony Forming Units (CFU). Data were analysed by One Way Anova test followed by a Tukey HSD test. Results: The MIC value at a concentration of 0.781% and the MBC value at a concentration of 1.56%. In positive control, there was an average bacterial death of 0%, a concentration of 0.781% had an average bacterial death of 91%, and at a concentration of 1.56% the average bacterial death was 100%. The results showed that the greater the concentration of nano anchovy, the stronger the antibacterial power. Conclusion: There is antibacterial potency in nano brown anchovy against Mixed Bacteria in deep dentin caries lesions.

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Section: Introductionmentioning

confidence: 99%

Antibacterial Power of Nano Brown Anchovy (<i>Stolephorus insularis</i>) Against Mixed Bacteria in Deep Dentin Caries

Yuanita

Paramitha

Mustamu

et al. 2023

IJMDS

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Inhibition of Streptococcus mutans biofilm formation using combination of nano brown anchovy (Stolephorus insularis) and calcium hydroxide

Yuanita,

Sukaton,

Paramitha

et al. 2023

CDJ

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Background: One of the important virulence factors of S. mutans is the biofilm formation. Some bacteria in biofilms show resistance to antimicrobials. Therefore, the addition of nano brown anchovy (S. insularis) as antibiofilm agents can potentiate the caries and infection prevention efficacy of endodontic treatment. Purpose: To describe the effect of the combination of nano brown anchovy (S. insularis) 12% and Ca(OH)2 with a ratio of 1:1 on inhibition of S. mutans biofilm formation. Methods: Laboratory experimental research on S. mutans bacteria was carried out in vitro using the crystal violet assay method. Brown anchovy was made into nanoparticles, dissolved to a concentration of 12%, and combined with Ca(OH)2. Biofilm samples were given treatment and observed for inhibition of biofilm growth. Biofilm growth inhibition was seen from the Optical Density (OD)540nm absorbance value measured using a spectrophotometer. Results: In the group that was tested with Ca(OH)2, the average percentage in S. mutans biofilm reduction was 55%, while in the group that was tested with a combination of nano brown anchovy (S. insularis) and Ca(OH)2, the average percentage in S. mutans biofilm reduction was 61%. Conclusion: The combination of nano brown anchovy (S. insularis) 12% and Ca(OH)2 with a ratio of 1:1 can inhibit the formation of S. mutans biofilm.

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Effect of combination of nano brown anchovy (Stolephorus insularis) nanoparticles and calcium hydroxide on inhibition Streptococcus sanguinis biofilm formation

Yuanita,

Juniarti,

Nabilla

et al. 2023

CDJ

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Background: Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans are gram bacteria that can cause various problems in the oral cavity so that materials that have antibacterial potency are needed. Calcium hydroxide, chlorhexidine, and sodium hypochlorite as antibacterial ingredients have disadvantages such as forming tunnel defects, causing dysgeusia, and damaging periapical tissue, so it is hoped that there will be natural materials as alternatives. The nano brown anchovy has fluor as the active compound which has the potential as an antibacterial agent. Purpose: To analyze the effect of antibacterial potency of nano brown anchovy (Stolephorus insularis) on Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans. Methods: This research is a laboratory experimental in vitro with the post-test only control group design. Brown anchovy is made into nano and diluted by dilution method into several concentrations. The direct contact method was used between some concentrations and the two bacteria. The values of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Kill Concentration) were obtained by counting the number of bacterial colonies on Mueller Hinton Agar media. Bacterial colony growth was calculated manually in Colony Forming Units (CFU). Results: Antibacterial potency of nano brown anchovy on Lactobacillus acidophilus showed MIC results at 1.56% concentration and MBC at 3.125% concentration. Antibacterial potency of nano brown anchovy on Aggregatibacter actinomycetemcomitans showed MIC results at 3.125% concentration and MBC at 6.25% concentration. Conclusion: Nano brown anchovy has antibacterial activity on Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans.

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The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

Cited by 3 publications

References 7 publications

Antibacterial Power of Nano Brown Anchovy (<i>Stolephorus insularis</i>) Against Mixed Bacteria in Deep Dentin Caries

Antibacterial Power of Nano Brown Anchovy (<i>Stolephorus insularis</i>) Against Mixed Bacteria in Deep Dentin Caries

Inhibition of Streptococcus mutans biofilm formation using combination of nano brown anchovy (Stolephorus insularis) and calcium hydroxide

Effect of combination of nano brown anchovy (Stolephorus insularis) nanoparticles and calcium hydroxide on inhibition Streptococcus sanguinis biofilm formation

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