The effectiveness of biogenic silver nanoparticles in the treatment of caprine mastitis induced by Staphylococcus aureus

Abstract

The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammary inoculated with 1.5×10 8 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1 st group left without treatment as control positive, 2 nd group treated intramammary infusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3 rd group treated intramammary infusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4 th group treated intramammary infusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14-and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

“…The oval to elliptical form of the granular particles indicates that the natural architecture of β-glucan particles has been preserved, as mentioned by Raikos et al (2018) . In yeast cells, the FTIR spectrum was utilized to analyze three major sections, which correspond to polysaccharides (925–1,190 cm –1 ), proteins (1,500–1,700 cm –1 ), and lipids (2,800–3,000 cm –1 ), ( Liu et al, 2015 ; Pengkumsri et al, 2017 ; Al Dujaily and Mahmood, 2021 ).…”

Review on mastitis and public health importance of mastitis causing pathogens in raw milk from Mastitis infected dairy goat.

“…There are many microorganisms that cause mastitis in goats [37]. Unappropriate milking tecniques and unsuitable hygiene conditions increases the infections (38).…”