In organised industrial zones, where workplaces of different hazard classes operate, the risk of injury is high. Therefore, it is important that workers, who are prone to accidents, are prepared to intervene in medical emergencies. This study aimed to evaluate the first aid knowledge levels of industry workers and related factors. This descriptive and cross-sectional study was conducted with 444 industrial workers employed in different institutions in an organised industrial zone in a Turkish province. The data were collected using the descriptive information and first aid knowledge forms. The total score first aid knowledge level of the industrial workers who participated in the study was found to be 19.62 ± 6.02. The findings revealed that the first aid knowledge levels of the industrial workers aged between 36 and 45, married, graduates of high school or a higher educational institution, tenured for more than 1096 days or higher and recipients of first aid training were higher (p