The Effectiveness of Triamcinolone Injection on Risk of Postoperative Operations with the Conjunctiva Autograft Technique and Its Association with Change of VEGF mRNA Expression

Purnamanita, Purnamanita; Budu, Budu; Hatta, Mochammad; Massi, Maurizio; Natzir, Rosdiana; Ichsan, Andi Muhammad; Sujuti, Hidayat

doi:10.13005/bpj/1916

Cited by 7 publications

(2 citation statements)

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“…The PCR program is then ready to run on a Realtime PCR machine (CFX Connect system, Biorad Laboratories, Real Time PCR 96 well 0.1 mL, USA). The value of ΔCt will be determined by subtracting the target Ct of each sample from the Ct value of its ß –actin [ 21 , [23] , [24] , [25] ] .…”

Section: Methodsmentioning

confidence: 99%

Correlation between innate and adaptive immunity response in TB children post BCG vaccination. Is it effective or not?: Cross-sectional study

Shabariah

Farsida

Hatta

et al. 2022

Annals of Medicine &Amp; Surgery

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Section: Methodsmentioning

confidence: 99%

Correlation between innate and adaptive immunity response in TB children post BCG vaccination. Is it effective or not?: Cross-sectional study

Shabariah

Farsida

Hatta

et al. 2022

Annals of Medicine &Amp; Surgery

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“…The mRNA concentration was measured with a QubitTM fluorometer (Invitrogen, Carlsbad, CA, United States) according to the manufacturer's 90 instructions, and the RNA samples were then stored at −80 °C until the next analysis. Examination of expression mRNA gene using the nucleic acid extraction method, expression mRNA gene Treg using the Real-Time Polymerase Chain Reaction (RT PCR) method, calculating the calibration curve with Ct (Cycle threshold) [ [12] , [13] , [14] , [15] ].…”

Section: Methodsmentioning

confidence: 99%

Relationship between expression mRNA gene Treg, Treg, CD4+, and CD8+ protein levels with TST in tuberculosis children: A nested case-control

Farsida

Shabariah

Hatta

et al. 2021

Annals of Medicine and Surgery

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Background The ability of Mycobacterium tuberculosis to survive intracellularly, provides a cellular adaptive immune response played by specific T cells to defend against tuberculosis. The adaptive immune response to Bacillus of Calmette and Guerin (BCG) immunization is responded to by B cells, T Follicular B helper, T regulatory, restriction CD1, CD8 + , CD4 + , Th1, Th2, and Th17. BCG immunization can cause a tuberculin test reaction to being positive. The tuberculin test is a method for diagnosing TB infection and for screening individuals for latent infection and assessing the rate of TB infection in a given population. Methods a nested case-control survey was conducted on patients with a diagnosis of TB and parents 0–18 years of age from 3 hospitals in Indonesia during September–November 2019 with a total sample of 69 people undergoing clinical examinations, supporting and diagnosing subjects, blood sampling 1–2 cc for examination mRNA gene Treg, Treg, CD 4+, and CD 8+, then centrifuged at 3000 rpm for 10 min to support blood cells and serum. Results There was a significant relationship between expression of mRNA gene Treg with TST (p = 0,000), Treg with TST (p = 0,000), and CD4 + with TST (p = 0,000). Meanwhile, CD8 + was not significantly associated with TST (p = 0.118). Conclusions It is necessary to check the expression of mRNA gene Treg, Treg, CD4 + , and CD8 + with more samples to find the mean value that shows the protective value of further TB.

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Effect of breastfeeding on children's health and its relationship to NRAMP1 expression: A cross-sectional study

Fatimah

Massi

Febriani

et al. 2021

Annals of Medicine and Surgery

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The Effectiveness of Triamcinolone Injection on Risk of Postoperative Operations with the Conjunctiva Autograft Technique and Its Association with Change of VEGF mRNA Expression

Cited by 7 publications

References 0 publications

Correlation between innate and adaptive immunity response in TB children post BCG vaccination. Is it effective or not?: Cross-sectional study

Correlation between innate and adaptive immunity response in TB children post BCG vaccination. Is it effective or not?: Cross-sectional study

Relationship between expression mRNA gene Treg, Treg, CD4+, and CD8+ protein levels with TST in tuberculosis children: A nested case-control

Effect of breastfeeding on children's health and its relationship to NRAMP1 expression: A cross-sectional study

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