The Effectors of Smut Fungi

Doehlemann, Gunther; Schipper, Kerstin; Kahmann, Regine

doi:10.1002/9781119949138.ch4

Cited by 1 publication

(1 citation statement)

References 69 publications

(109 reference statements)

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“…Recently, expression of the U. maydis effector Pit2 (93 aa), was identified as a requirement for tumour formation (Doehlemann et al ., 2011a). U. maydis effector proteins typically have a secretion signal, are upregulated during in planta growth, and lack similarity to known proteins (reviewed in Doehlemann et al ., 2011b). Predicted proteins for eight U. maydis NATs contain recognizable secretion signals.…”

Section: Discussionmentioning

confidence: 99%

Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis

Donaldson¹,

Saville

2013

Molecular Microbiology

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Ustilago maydis infection of Zea mays leads to the production of thick-walled diploid teliospores that are the dispersal agent for this pathogen. Transcriptome analyses of this model biotrophic basidiomycete fungus identified natural antisense transcripts (NATs) complementary to 247 open reading frames. The U. maydis NAT cDNAs were fully sequenced and annotated. Strand-specific RT-PCR screens confirmed expression and identified NATs preferentially expressed in the teliospore. Targeted screens revealed four U. maydis NATs that are conserved in a related fungus. Expression of NATs in haploid cells, where they are not naturally occurring, resulted in increased steady-state levels of some complementary mRNAs. The expression of one NAT, as-um02151, in haploid cells resulted in a twofold increase in complementary mRNA levels, the formation of sense–antisense double-stranded RNAs, and unchanged Um02151 protein levels. This led to a model for NAT function in the maintenance and expression of stored teliospore mRNAs. In testing this model by deletion of the regulatory region, it was determined that alteration in NAT expression resulted in decreased pathogenesis in both cob and seedling infections. This annotation and functional analysis supports multiple roles for U. maydis NATs in controlling gene expression and influencing pathogenesis.

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Section: Discussionmentioning

confidence: 99%