2012
DOI: 10.1016/j.ygyno.2011.08.026
|View full text |Cite
|
Sign up to set email alerts
|

The effects of 17β-estradiol and a selective estrogen receptor modulator, bazedoxifene, on ovarian carcinogenesis

Abstract: Objective To test if estrogen promotes carcinogenesis in vitro and in a genetic mouse model of ovarian cancer and whether its effects can be inhibited by a novel selective estrogen receptor modulator (SERM), bazedoxifene. Methods Bazedoxifene was synthesized and it was confirmed that the drug abrogated the uterine stimulatory effect of 17β-estradiol in mice. To determine if hormones alter tumorigenesis in vivo LSL-K-rasG12D/+PtenloxP/loxP mice were treated with vehicle control, 17β-estradiol or bazedoxifene.… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
12
0

Year Published

2012
2012
2019
2019

Publication Types

Select...
6

Relationship

3
3

Authors

Journals

citations
Cited by 10 publications
(12 citation statements)
references
References 43 publications
0
12
0
Order By: Relevance
“…Quantification of immunostaining was performed using the Automated Cellular Imaging System (ACIS, Clarient, San Juan Capistrano, CA) by setting color-specific thresholds to determine brown (positive) and blue (negative) nuclei within 12 representative regions per slide and by calculating the ratio of positively stained nuclei to all nuclei, expressed as a region score. 19,22 …”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Quantification of immunostaining was performed using the Automated Cellular Imaging System (ACIS, Clarient, San Juan Capistrano, CA) by setting color-specific thresholds to determine brown (positive) and blue (negative) nuclei within 12 representative regions per slide and by calculating the ratio of positively stained nuclei to all nuclei, expressed as a region score. 19,22 …”
Section: Methodsmentioning
confidence: 99%
“…The LSL- K-ras G12D/+ PTEN loxP/loxP genetic mouse model was obtained from the Massachusetts Institute of Technology (Boston, MA) 24 and used as previously described. 17,22 OvCa was initiated by intrabursal injection of AdCre virus (2.5×10 7 plaque-forming units) in the right ovary; the left ovary was not injected and served as an internal control. Two weeks after initiation of OvCa, treatment began with control, paclitaxel 3 mg/kg/week injected i.p., or metformin added to water for an estimated final dose of 100 mg/kg/day per mouse.…”
Section: Methodsmentioning
confidence: 99%
“…OvCa cells were treated with varying concentrations of lovastatin (10 µM, 20 µM and 40 µM) for 24 hours, and the rate of cellular proliferation was measured as previously described [36]. Lovastatin was purchased from Cayman Chemical (Ann Arbor, MI).…”
Section: Methodsmentioning
confidence: 99%
“…Chang et al, (2010) demonstrated that BZA antagonized estrogen‐mediated increases in proliferation and gene expression of a human estrogen‐responsive breast cancer cell line (MCF‐7). Romero et al (2012) characterized the effect of BZA on ERα and ERβ expression levels in ovarian tumors and determined that ERα expression was significantly decreased in tumors from mice given BZA compared with controls. In contrast, treatment of mice with estradiol or BZA resulted in significantly lower or higher levels of ERβ expression in tumors, respectively.…”
Section: Discussionmentioning
confidence: 99%
“…Considering the role that HRT and estrogens play in development and progression of ovarian cancer, Romero et al (2012) recently assessed the ability of estrogens to promote carcinogenesis in vitro and in a genetic mouse model of ovarian cancer and whether its effects could be inhibited by BZA. The authors reported that while estradiol increased both the invasion and proliferation of ovarian cancer cells in vitro, and BZA reversed these effects, neither treatment with estradiol nor BZA changed mean tumor burden when compared with treatment with placebo in the LSL‐K‐ras G12D/+ Pten loxP/loxPa model of endometrioid ovarian cancer.…”
Section: Discussionmentioning
confidence: 99%