The effects of 2‐hydroxyethyl methacrylate on matrix metalloproteinases 2 and 9 in human pulp cells and odontoblast‐like cells <i>in vitro</i>

Sun, Shuming; Wang, G.-l.; Huang, Ying; Diwu, H.-l.; Luo, Youcheng; Su, Jun; Xiao, Yuhong

doi:10.1111/iej.12812

Cited by 11 publications

(10 citation statements)

References 39 publications

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“…Comparatively, due to the lack of acidity in the latter study, this author attributed the observed effect to the monomer content [23]. Interestingly, the current findings are in contradiction with those of Sun et al who found HEMA, another leachable monomer, to inhibit MMP-2 and MMP-9 expression in pulp cells, thereby suggesting a potential hybrid-layer protective role to this constituent [25].…”

Section: Discussioncontrasting

confidence: 92%

“…Following extraction, pulp tissue was removed according to Sun et al [25]. Through an explant method, cells were cultured in minimum essential medium Eagle-alpha modification (Alpha MEM) containing ultraglutamine 1, ribonucleosides, and deoxyribonucleosides (Lonza, Basel, Switzerland) with the addition of 10% fetal bovine serum (FBS, Euroclone, Milan, Italy) and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 2.5 μg/ml amphotericin B) and cultured in a humidified atmosphere containing 5% CO 2 at 37°C.…”

Section: Pulp Cell Culturementioning

confidence: 99%

“…TEGDMA has been shown to inhibit the activity of MMP-2 extracted from mouse gingival tissue [24]. While, hydroxyethyl methacrylate (HEMA) inhibited MMP-2 and MMP-9 activity and production in pulp-derived cells [25]. Few gene expression studies have found MMP-1, MMP-3, MMP-10, and MMP-12 increases in pulp cells upon exposure to low toxic TEGDMA concentrations [26,27].…”

Section: Introductionmentioning

confidence: 99%

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Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells

et al. 2020

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Objectives Resin-based composites may leach monomers such as triethylene-glycol dimethacrylate (TEGDMA), which could contribute to intrapulpal inflammation. The aim of this investigation was to examine whether various concentrations of TEGDMA are able to influence dentally relevant Matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 production, total collagenase/gelatinase activity in pulp cells, and suggest possible signaling mechanisms. Materials and methods Pulp cells were cultured, followed by a 1-day exposure to sublethal TEGDMA concentrations (0.1, 0.2, and 0.75 mM). Total MMP activity was measured by an EnzCheck total collagenase/gelatinase assay, while the production of specific MMPs and the relative changes of phosphorylated, i.e., activated signaling protein levels of extracellular signal-regulated kinase (ERK)1/2, p38, c-Jun N-terminal kinase (JNK) were identified by western blot. Immunocytochemistry image data was also plotted and analyzed to see whether TEGDMA could possibly alter MMP production. Results An increase in activated MMP-2, MMP-8, and MMP-9 production as well as total collagenase activity was seen after a 24-h exposure to the abovementioned TEGDMA concentrations. Increase was most substantial at 0.1 (P = 0.002) and 0.2 mM (P = 0.0381). Concurrent pERK , p-p38, and p-JNK elevations were also detected. Conclusions Results suggest that monomers such as TEGDMA, leached from resin-based restorative materials, activate and induce the production of dentally relevant MMPs in pulp cells. Activation of ERK1/2, p38, or JNK and MMP increase may play a role in and/or can be part of a broader stress response. Clinical relevance Induction of MMP production and activity may further be components in the mechanisms of intrapulpal monomer toxicity.

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Section: Discussioncontrasting

confidence: 92%

Section: Pulp Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells

et al. 2020

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“…Pulp tissue was isolated from caries-free third molar teeth extracted for orthodontic reasons from two individuals. Following extraction, pulp tissue was immediately isolated [ 21 ] and cultured through an explant method. For culturing minimum essential medium eagle-alpha modification (Alpha MEM) containing nucleosides, routinely supplemented with 1 mM stable Glutamine (Capricorn Scientific GmbH, Ebsdorfergrund, Germany), 10% fetal bovine serum (FBS, Euroclone, Milan, Italy), and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin, 2.5 μg/mL amphotericin B) was used.…”

Section: Methodsmentioning

confidence: 99%

Does Preheating Influence the Cytotoxic Potential of Dental Resin Composites?

Dunavári,

Kőházy,

Vecsernyés

et al. 2024

Polymers

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Resin-based dental composites (RBC) release cytotoxic components, however the extent of the elution from preheated RBCs is barely investigated. The aim was therefore to determine the cytotoxic effect of preheated conventional, bulk, and thermoviscous RBCs of clinically relevant sizes using different cell viability methods in a contact-free model. Samples (6 × 4 mm) were prepared from conventional [Estelite Sigma Quick (ESQ), Filtek Z250 (FZ)] and bulk-filled [Filtek One BulkFill Restorative (FOB), SDR Plus Bulk Flow (SDR), VisCalor Bulk (VCB)] RBCs. The pre-polymerization temperature was set to room temperature (RT) and 55/65 °C. Pulp cells were cultured, followed by a 2-day exposure to monomers released from solid RBC specimens suspended in the culture medium. Cytotoxicity was assessed using a WST-1, MTT, and LDH colorimetric viability assays. Data were analyzed using one-way ANOVA, Tukey’s post hoc test, multivariate analysis, and independent t-test. The effect size (ƞp2) of material and temperature factors was also assessed. All the RBCs demonstrated cytotoxic effect upon exposure to pulp cells, but to a varying extent (ESQ >> VCB > FZ = FOB = SDR). The effect of pre-polymerization temperature was insignificant (ƞp2 < 0.03), except for the thermoviscous RBC, which showed inconsistent findings when subjected to distinct viability tests. Cell viability was predominantly dependent on the type of material used (p < 0.001) which showed a large effect size (ƞp2 > 0.90). Irrespective of the pre-polymerization temperature, RBC samples in a clinically relevant size can release monomers to such an extent, which can substantially decrease the cytocompatibility.

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“…Hepatocellular carcinoma (HCC) is a primary tumour of the liver and several risk factors for its development have been identified. These include hepatitis C viral (HCV) infection, hepatitis B virus (HBV) infection, hereditary hemochromatosis and cirrhosis of almost any cause[ 2 ].…”

Section: Introductionmentioning

confidence: 99%

MR with Gd-EOB-DTPA in assessment of liver nodules in cirrhotic patients

Inchingolo

Faletti

Grazioli

et al. 2018

WJH

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To date the imaging diagnosis of liver lesions is based mainly on the identification of vascular features, which are typical of overt hepatocellular carcinoma (HCC), but the hepatocarcinogenesis is a complex and multistep event during which, a spectrum of nodules develop within the liver parenchyma, including benign small and large regenerative nodule (RN), low-grade dysplastic nodule (LGDN), high-grade dysplastic nodule (HGDN), early HCC, and well differentiated HCC. These nodules may be characterised not only on the basis of their respective different blood supplies, but also on their different hepatocyte function. Recently, in liver imaging the introduction of hepatobiliary magnetic resonance imaging contrast agent offered the clinicians the possibility to obtain, at once, information not only related to the vascular changes of liver nodules but also information on hepatocyte function. For this reasons this new approach becomes the most relevant diagnostic clue for differentiating low-risk nodules (LGDN-RN) from high-risk nodules (HGDN/early HCC or overt HCC) and consequently new diagnostic algorithms for HCC have been proposed. The use of hepatobiliary contrast agents is constantly increasing and gradually changing the standard of diagnosis of HCC. The main purpose of this review is to underline the added value of Gd-EOB-DTPA in early-stage diagnoses of HCC. We also analyse the guidelines for the diagnosis and management of HCC, the key concepts of HCC development, growth and spread and the imaging appearance of precursor nodules that eventually may transform into overt HCC.

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The effects of 2‐hydroxyethyl methacrylate on matrix metalloproteinases 2 and 9 in human pulp cells and odontoblast‐like cells in vitro

Abstract: Cell viability was not affected at HEMA concentrations ≤400 μg mL . Within this range, HEMA inhibited MMP-2 and MMP-9 expression and activity, which may protect against type I collagen degradation effectively during dentine adhesive procedures.

Cited by 11 publications

References 39 publications

Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells

Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells

Does Preheating Influence the Cytotoxic Potential of Dental Resin Composites?

MR with Gd-EOB-DTPA in assessment of liver nodules in cirrhotic patients

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