The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells

Fan, Jingzhang; Bi, Zhengang

doi:10.1590/1414-431x20154494

Cited by 30 publications

(29 citation statements)

References 34 publications

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“…6G potentiated the toxic effect of cisplatin by amplifying ROS generation. The ability of 6G to enhance intracellular accumulation of cisplatin by reducing its efflux agrees with previous studies that natural agents reverse multidrug resistance involved in regulating the efflux of chemotherapeutic drugs from cells [ 50 - 52 ]. It is possible that 6G exacerbated cisplatin-induced apoptotic cell death in cervical cancer cells by coupling enhanced oxidative stress with increased accumulation of cisplatin.…”

Section: Discussionsupporting

confidence: 88%

Proteasome inhibition mediates p53 reactivation and anti-cancer activity of 6-Gingerol in cervical cancer cells

et al. 2015

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Human papilloma virus (HPV) expressing E6 and E7 oncoproteins, is known to inactivate the tumor suppressor p53 through proteasomal degradation in cervical cancers. Therefore, use of small molecules for inhibition of proteasome function and induction of p53 reactivation is a promising strategy for induction of apoptosis in cervical cancer cells. The polyphenolic alkanone, 6-Gingerol (6G), present in the pungent extracts of ginger (Zingiber officinale Roscoe) has shown potent anti-tumorigenic and pro-apoptotic activities against a variety of cancers. In this study we explored the molecular mechanism of action of 6G in human cervical cancer cells in vitro and in vivo. 6G potently inhibited proliferation of the HPV positive cervical cancer cells. 6G was found to: (i) inhibit the chymotrypsin activity of proteasomes, (ii) induce reactivation of p53, (iii) increase levels of p21, (iv) induce DNA damage and G2/M cell cycle arrest, (v) alter expression levels of p53-associated apoptotic markers like, cleaved caspase-3 and PARP, and (vi) potentiate the cytotoxicity of cisplatin. 6G treatment induced significant reduction of tumor volume, tumor weight, proteasome inhibition and p53 accumulation in HeLa xenograft tumor cells in vivo. The 6G treatment was devoid of toxic effects as it did not affect body weights, hematological and osteogenic parameters. Taken together, our data underscores the therapeutic and chemosensitizing effects of 6G in the management and treatment of cervical cancer.

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Section: Discussionsupporting

confidence: 88%

Proteasome inhibition mediates p53 reactivation and anti-cancer activity of 6-Gingerol in cervical cancer cells

et al. 2015

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“…In a similar fashion, Fan, Yang, and Bi () investigated the effect of [6]‐gingerol on the production of IL‐6 in osteoblasts. Results revealed that [6]‐gingerol lowers the degree of inflammation in TNF‐α‐treated MG‐63 cells.…”

Section: Resultsmentioning

confidence: 99%

“…The protective effect of [6]-gingerol with the IL-1β-induced inflammatory response is similar to that of butylated hydroxytoluene, an ROS scavenger. Thus [6]-gingerol could protect HuH7 cells against inflammatory damage induced by IL-1β by inhibiting the ROS/NF-κB/COX-2 pathway.In a similar fashion,Fan, Yang, and Bi (2015) investigated the effect of [6]-gingerol on the production of IL-6 in osteoblasts. Results revealed that [6]-gingerol lowers the degree of inflammation in TNF-αtreated MG-63 cells.…”

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confidence: 99%

Protective and therapeutic potential of ginger (Zingiber officinale) extract and [6]‐gingerol in cancer: A comprehensive review

Lima

Reis

Menezes

et al. 2018

Phytotherapy Research

197

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Natural dietary agents have attracted considerable attention due to their role in promoting health and reducing the risk of diseases including cancer. Ginger, one of the most ancient known spices, contains bioactive compounds with several health benefits. [6]-Gingerol constitutes the most pharmacologically active among such compounds. The aim of the present work was to review the literature pertaining to the use of ginger extract and [6]-gingerol against tumorigenic and oxidative and inflammatory processes associated with cancer, along with the underlying mechanisms of action involved in signaling pathways. This will shed some light on the protective or therapeutic role of ginger derivatives in oxidative and inflammatory regulations during metabolic disturbance and on the antiproliferative and anticancer properties. Data collected from experimental (in vitro or in vivo) and clinical studies discussed in this review indicate that ginger extract and [6]-gingerol exert their action through important mediators and pathways of cell signaling, including Bax/Bcl2, p38/MAPK, Nrf2, p65/NF-κB, TNF-α, ERK1/2, SAPK/JNK, ROS/NF-κB/COX-2, caspases-3, -9, and p53. This suggests that ginger derivatives, in the form of an extract or isolated compounds, exhibit relevant antiproliferative, antitumor, invasive, and anti-inflammatory activities.

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“…Moreover, the hexane extract of ginger inhibited RANKL-induced osteoclastogenesis [ 15 ]. Gingerol significantly enhanced the proliferation, differentiation, and mineralization of osteoblast-like MG-63 cells [ 16 ]. A. melegueta K. Schum.…”

Section: Introductionmentioning

confidence: 99%

Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells

et al. 2017

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Osteoporosis is a serious health problem characterized by decreased bone mineral density and deterioration of bone microarchitecture. Current antiosteoporotic agents exhibit a wide range of adverse effects; meanwhile, phytochemicals are effective and safer alternatives. In the current work, nine compounds belonging to hydroxyphenylalkane and diarylheptanoid groups were isolated from Aframomum meleguea seeds and identified as 6-gingerol (1), 6-paradol (2), 8-dehydrogingerdione (3), 8-gingerol (4), dihydro-6-paradol (5), dihydrogingerenone A (6), dihydrogingerenone C (7), 1,7-bis(3,4-dihydroxy-5-methoxyphenyl)heptane-3,5-diyl diacetate (8), and 1-(3,4-dihydroxy-5-methoxyphenyl)-7-(3,4-dihydroxyphenyl)heptane-3,5-diyl diacetate (9). The structures of isolated compounds were established by NMR and mass spectral data, in addition to referring to literature data. Exposure of MCF-7, MG-63, and SAOS-2 cells to subcytotoxic concentrations of the compounds under investigation resulted in accelerated proliferation. Among them, paradol was selected for further detailed biochemical analysis in SAOS-2 cells. DNA flowcytometric analysis of cell cycle distribution revealed that paradol did not induce any significant change in the proliferation index of SAOS-2 cells. Assessment of osteogenic gene expression revealed that paradol enhanced the expression of osteocyte and osteoblast-related genes and inhibited osteoclast and RUNX suppressor genes. Biochemically, paradol enhanced alkaline phosphatase activity and vitamin D content and decreased the osteoporotic marker acid phosphatase. In conclusion, paradol, which is a major constituents of A. melegueta seeds, exhibited potent proliferative and ossification characteristics in bone cells.

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The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells

Cited by 30 publications

References 34 publications

Proteasome inhibition mediates p53 reactivation and anti-cancer activity of 6-Gingerol in cervical cancer cells

Proteasome inhibition mediates p53 reactivation and anti-cancer activity of 6-Gingerol in cervical cancer cells

Protective and therapeutic potential of ginger (Zingiber officinale) extract and [6]‐gingerol in cancer: A comprehensive review

Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells

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