The effects of a low dose OTA exposure on weanling piglet gut microbiota

Grosu, Iulian Alexandru; Cişmileanu, Ana; Marin, Daniela Eliza; Ţăranu, Ionelia

doi:10.2478/azibna-2021-0003

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…Standard curves were generated using serial dilutions of template DNA (105–1012 molecules/µL). The selective bacteria genera DNA was quantified using the RotorGene Q series PCR system (Qiagen, Hilden, Germany) with species-specific primers sequences designed for the following genera: Lactobacillus , Clostridium , Enterobacter , Prevotella and Bifidobacterium and using a PCR protocol already described elsewhere [ 80 , 81 ]. The gene copy numbers for the selected microorganisms were obtained using the Cq values relative to the standard curve.…”

Section: Methodsmentioning

confidence: 99%

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Grosu

Bulgaru

Pistol

et al. 2023

Toxins

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Pigs are the most sensitive animal to zearalenone (ZEN) contamination, especially after weaning, with acute deleterious effects on different health parameters. Although recommendations not to exceed 100 µg/kg in piglets feed exists (2006/576/EC), there are no clear regulations concerning the maximum limit in feed for piglets, which means that more investigations are necessary to establish a guidance value. Due to these reasons, the present study aims to investigate if ZEN, at a concentration lower than the EC recommendation for piglets, might affect the microbiota or induce changes in SCFA synthesis and can trigger modifications of nutritional, physiological, and immunological markers in the colon (intestinal integrity through junction protein analysis and local immunity through IgA production). Consequently, the effect of two concentrations of zearalenone were tested, one below the limit recommended by the EC (75 µg/kg) and a higher one (290 µg/kg) for comparison reasons. Although exposure to contaminated feed with 75 µg ZEN/kg feed did not significantly affect the observed parameters, the 290 µg/kg feed altered several microbiota population abundances and the secretory IgA levels. The obtained results contribute to a better understanding of the adverse effects that ZEN can have in the colon of young pigs in a dose-dependent manner.

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Section: Methodsmentioning

confidence: 99%

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Grosu

Bulgaru

Pistol

et al. 2023

Toxins

Self Cite

View full text Add to dashboard Cite

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The Use of Agro-Industrial Waste Rich in Omega-3 PUFA during the Weaning Stress Improves the Gut Health of Weaned Piglets

et al. 2022

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(1) Background: The weaning period is a very important stage in the pig life, as during weaning, the animals are very susceptible to pathogens and develop postweaning diarrhoea. The aim of our study was to counteract weaning stress and to improve piglets’ gut health by using a nutritional intervention consisting of a mix of agro-industrial wastes (grapeseed, flaxseed and sea blackthorn meals) rich in omega-3 PUFA. (2) Methods: Twelve cross-bred TOPIG hybrid piglets with an average body weight of 11.25 kg were randomly distributed to one of the two experimental groups: a control group fed basic corn soybean diet (control diet) and an experimental group fed a diet with a 10% mixture of grapeseed, flaxseed and sea buckthorn meals in a ratio of 3:4:1 (GFS diet). (3) Results: the GFS diet had no effect on the performance, biochemical parameters or the total antibody synthesis. GFS diet was able to significantly reduce the concentration of proinflammatory cytokines IL-1 beta and TNF-alpha and to significantly increases the expression of junction proteins (occludin, claudin 4, claudin 7 and extracellular protein matrix) at the gene or protein level as compared with control. The presence of GFS in the diet increased the abundance of Bifidobacterium and Lactobacillus species in the colonic content as well as the concentration of propionic and butyric acids. (4) Conclusions: Taken together, our results showed that agro-industrial wastes rich in omega-3 PUFA can be used as an ecological, environmentally friendly nutritional intervention for improving the negative effects associated with the weaning stress.

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The effects of a low dose OTA exposure on weanling piglet gut microbiota

Cited by 2 publications

References 49 publications

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

The Use of Agro-Industrial Waste Rich in Omega-3 PUFA during the Weaning Stress Improves the Gut Health of Weaned Piglets

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