The Effects of a Probiotic (Bacillus clausii) in Acute Kidney Injury in a Rat Model of LPS-Induced Endotoxemia

Abstract

Objective: This study aims to examine the role of a probiotic bacterium, Bacillus clausii, on oxidative stress in a lipopolysaccharide (LPS)-induced acute kidney injury (AKI) rat model. Materials and Methods:The rats were divided into four groups: Control group, LPS group (1.5 mg/kg, LPS), Probiotic+LPS group in which LPS was given after administration of Bacillus clausii as a probiotic for 21 days, and Probiotic group. The kidneys of the rats were removed 24 hours after LPS injection. Total antioxidant status, total oxidant status, oxidative stress index, malondialdehyde and myeloperoxidase (MPO) values were biochemically determined in the kidneys. Furthermore, the kidney tissue samples were immunohistochemically stained for interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α expression, and leukocyte distribution.Results: Endotoxemia caused an increase in oxidative stress (p<0.001), lipid peroxidation (p<0.01), MPO activity (p<0.001), and the expression of IL-6 (p<0.001) and TNF-α (p<0.001). The administration of probiotic ameliorated oxidative stress, lipid peroxidation, and myeloperoxidase activity, and resulted in decreased IL-6 and TNF-α reactions that were elevated with LPS treatment. Conclusion:The results suggest that Bacillus clausii as a probiotic bacterium may have an antioxidative property in LPSinduced AKI.