The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

Andriani, Dwi; Pargaputri, Agni Febrina

doi:10.20473/j.djmkg.v51.i4.p205-209

Cited by 3 publications

(8 citation statements)

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“…18 Previous studies have shown that A. ilicifolius extract has antifungal and antioxidant effects in vitro and antifungal activity in mice suffering from oral candidiasis under immunosuppressed conditions. [12][13][14] Cell death was not significantly different between the nystatin and 20% A. ilicifolius extract treatment groups. This shows that A. ilicifolius therapy has the same efficacy as nystatin.…”

Section: Discussionmentioning

confidence: 96%

“…20 These secondary metabolites of A. ilicifolius extract contribute to its antifungal property by increasing the expression of Toll-like receptor 2, IL-22, and P38 in immunosuppressed rats with oral candidiasis. 3 13 14 These three markers are known to play a role in the inflammatory mechanism against C. albicans infection.…”

Section: Discussionmentioning

confidence: 99%

“…Each rat was then sacrificed, and the tongue was biopsied. 13,14 Cell Death Measurement Cell death was determined following staining with hematoxylin and eosin (Indo Reagen, AKD 10204600180, Indonesia). Measurement was made using a light microscope (Olympus CX-31) supported with an Optilab Viewer at 1,000Â magnification.…”

Section: Oral Candidiasis Treatmentmentioning

confidence: 99%

“…Between day 8 and day 20, the rat tongue was inoculated with 0.5 mL C. albicans ATCC 10231 three times a week for 2 weeks to induce oral candidiasis; McFarland 1 (3 Â 108 CFU/mL). 13,14 Examination of Hyphae and Yeast Hyphae were examined after staining with periodic acid-Schiff staining (Sigma, 395B, Sigma-Aldrich Pte Ltd, United States). Observations of the presence of yeast or hyphae on the upper epithelium were made with a light microscope (Olympus CX-31) supported by an Optilab Viewer at 400Â magnification.…”

Section: Induction Of Immunosuppressed Oral Candidiasismentioning

confidence: 99%

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Acanthus ilicifolius Methanolic Extract for Oral Candidiasis Treatment through Tongue Epithelial STAT3 and Cell Death Evaluation

et al. 2023

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Objectives This study aimed to evaluate the effects of topical application of Acanthus ilicifolius methanolic extract on signal transducer and activator of transcription 3 (STAT3) expression and tongue epithelial cell death caused by oral candidiasis under immunosuppressive conditions. Materials and Methods To obtain the oral candidiasis model, 20 healthy male Rattus norvegicus (Wistar) rats were administered oral dexamethasone and tetracycline for 14 days and oral candidiasis was induced with Candida albicans (ATCC-10231) 1 McFarland. They were then randomized into four groups—immunosuppression (K-), oral candidiasis (K + ), nystatin treatment (P1), and 20% A. ilicifolius methanolic extract treatment (P2) and were treated for 14 days. Histological analyses of cell death and candida invasion and immunohistochemical analysis of STAT3 in epithelial cells were performed. Statistical Analysis Epithelial cell death data were analyzed using one-way analysis of variance (ANOVA) and the post hoc Games–Howell test (p < 0.05) and STAT3 expression with one-way ANOVA and the post hoc least significant difference test (p < 0.05). Results Cell death was significantly different between K- and K+ and between K+ and P1 and P2 (p < 0.05); there were no significant differences between K- and P1 and P2 and between P1 and P2 (p > 0.05). STAT3 expression was significantly different between K- and P1 and P2 and between K+ and P1 and P2 (p < 0.05), but there were no significant differences between K+ and K- and between P1 and P2 (p > 0.05). Conclusion Topical administration of A. ilicifolius methanol extract increased STAT3 expression and decreased tongue epithelial cell death caused by oral candidiasis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Oral Candidiasis Treatmentmentioning

confidence: 99%

Section: Induction Of Immunosuppressed Oral Candidiasismentioning

confidence: 99%

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Acanthus ilicifolius Methanolic Extract for Oral Candidiasis Treatment through Tongue Epithelial STAT3 and Cell Death Evaluation

et al. 2023

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“…It is already known that Acanthus ilicifolius extract has antifungal and antioxidant in-vitro and became antifungal to mice that suffered from oral candidiasis 19,23,24 . This study confirmed that the immerse time in effervescent Acanthus ilicifolius extract 4% (Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic

Prananingrum

Rahmitasari

Andriani

et al. 2022

Odonto : Dental Journal

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Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.

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Identification of compounds isolated from a methanolic extract of Acanthus ilicifolius leaves and evaluation of their antifungal and antioxidant activity

2020

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Abstract. Andriani D, Revianti S, Prananingrum W. 2020. Identification of compounds isolated from a methanolic extract of Acanthus ilicifolius leaves and evaluation of their antifungal and antioxidant activity. Biodiversitas 21: 2521-2525. Acanthus ilicifolius L. (Acanthaceae) is commonly found in mangroves along the east coast of Surabaya. It can be used as an indicator of environmental pollution and damage in mangrove ecosystems. Studies have reported that A. ilicifolius has antimicrobial, antifungal, antiviral, anti-inflammatory, analgesic, antioxidant, anticancer, antileishmanial, and hepatoprotective activity due to the chemical compounds in the plant. This study aimed to determine the phytochemical compounds in methanolic extracts of A. ilicifolius and their antifungal and antioxidant activity. The study involved laboratory experiments with a post-test only control group design. Antifungal activity against Candida albicans biofilm was determined using microtiter plates. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Phytochemical screening used colorimetric methods. Methanolic extracts of A. ilicifolius at 16% and 20% concentration had the same inhibitory effect as nystatin against C. albicans (about 70% inhibition of biofilm). Chemical compounds identified in the extract included flavonoids, alkaloids, glycosides, polyphenols, tannins, and steroids. Methanolic extracts of A. ilicifolius have strong antioxidant and antifungal activity, and the plant’s phytochemical compounds are potential candidates for antifungal therapy.

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The effects of Acanthus ilicifolius chloroform extract on TLR-2 expression of macrophages in oral candidiasis

Cited by 3 publications

References 26 publications

Acanthus ilicifolius Methanolic Extract for Oral Candidiasis Treatment through Tongue Epithelial STAT3 and Cell Death Evaluation

Acanthus ilicifolius Methanolic Extract for Oral Candidiasis Treatment through Tongue Epithelial STAT3 and Cell Death Evaluation

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic

Identification of compounds isolated from a methanolic extract of Acanthus ilicifolius leaves and evaluation of their antifungal and antioxidant activity

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