The effects of acrylamide on brain creatine kinase: Inhibition kinetics and computational docking simulation

Lu, Ziwen; Zou, He-Chang; Park, Seong Jin; Park, Daeui; Shi, Long; Park, Yong‐Doo; Bhak, Jong; Zou, Fei

doi:10.1016/j.ijbiomac.2008.11.003

Cited by 9 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The time-dependent decrease of the enzyme activity of HBCK was best-fitted to a biphasic process, and the fast- ( k 1 ) and slow-phase ( k 2 ) rate constants are presented in Table 1. The changes in the transition free-energy (ΔΔ G °′) were also calculated by Equation 1 as described previously [30]:

Δ Δ G^{o}' = - R T ln k^{'}

where k′ is a time constant for the major phase (fast phase) of the inactivation reaction, T is the absolute temperature, and R is the Boltzmann constant. These results indicated that neither the inactivation rate constants nor ΔΔ G °′ were significantly affected by the different concentrations of acrylamide, and the values were at the same order of magnitude.…”

Section: Resultsmentioning

confidence: 99%

“…Both RBCK and HBCK exhibited a biphasic process during acrylamide-induced inactivation, but they had different rate constants [30]. However, only a monophasic inactivation process was discovered for RMCK [15].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies suggested that acrylamide can inhibit rabbit MM-CK (RMCK) and BB-CK (RBCK) by modifying the thiol groups of cysteines [15,30]. It is worth noting that BB-CK, but not MM-CK, is the predominant CK isoform in the brain and nervous system.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase

Sheng

Zou

et al. 2009

IJMS

Self Cite

View full text Add to dashboard Cite

Acrylamide is widely used worldwide in industry and it can also be produced by the cooking and processing of foods. It is harmful to human beings, and human brain CK (HBCK) has been proposed to be one of the important targets of acrylamide. In this research, we studied the effects of acrylamide on HBCK activity, structure and the potential binding sites. Compared to CKs from rabbit, HBCK was fully inactivated at several-fold lower concentrations of acrylamide, and exhibited distinct properties upon acrylamide-induced inactivation and structural changes. The binding sites of acrylamide were located at the cleft between the N- and C-terminal domains of CK, and Glu232 was one of the key binding residues. The effects of acrylamide on CK were proposed to be isoenzyme- and species-specific, and the underlying molecular mechanisms were discussed.

show abstract

Δ Δ G^{o}' = - R T ln k^{'}

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase

Sheng

Zou

et al. 2009

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Neurotoxicity is characterized by ataxia and skeletal muscle weakness. Numbness in hands and feet, fatigue and sweating of hands and feet are prominent symptoms of intoxication by this substance (Kopp and Dekant, 2009;Lu et al, 2009).…”

Section: Epidemiological Evidence Of Risk From Consumption Of Acrylammentioning

confidence: 99%

Estimated Acrylamide Intake from Coffee Consumption in Latin America

Porto¹,

Freitas-Silva²,

Rosa³

et al. 2015

American Journal of Agricultural and Biological Sciences

View full text Add to dashboard Cite

Abstract:The presence of acrylamide in foods such as coffee can pose risks to human health. Coffee consumption and production in Latin America have increased in recent years. According to the FAO, world coffee consumption is divided into five groups of countries according to average yearly per capita consumption: (i) between 8.0 kg and 12 kg; (ii) between 4.1 kg and 8.0 kg; (iii) between 2.4 kg and 4.5 kg; (iv) between 0.8 kg and 2.4 kg; and (v) less than 0.8 kg. In this work, the countries of Latin America are divided into three groups according to annual per capita coffee consumption: group 1: between 4.1 kg and 8.0 kg (Brazil, Argentina, Colombia and Costa Rica); group 2: between 2.4 kg and 4.5 kg (Guyana, French Guyana, Honduras, Suriname, Uruguay and Venezuela); and group 3: between 0.8 kg and 2.4 kg (Bolivia, Chile, Cuba, El Salvador, Ecuador, Guatemala, Haiti, Jamaica, Mexico, Nicaragua, Panama, Paraguay, Peru, Puerto Rico and Dominican Republic). There are great differences in the consumption of acrylamide-forming foods among populations from different countries. The literature shows a range from 0.5 to 4.21 µg of acrylamide per 300 mL of coffee and the World Health Organization recommends a maximum daily limit for acrylamide intake of 1 µg/kg bw. Thus, coffee is a beverage that contributes greatly to acrylamide intake in almost all population groups. From the calculated data on acrylamide intake in Latin America, it was possible to estimate the daily intake in the region's countries. According to the maximum limit set by the WHO of 1 µg acrylamide per day per bw, for people in the countries of group 1, coffee makes a large contribution to the intake of acrylamide. Thus, there should be focus on reducing the levels of acrylamide in foods, since they are the predominant sources of this substance for some populations.

show abstract

“…1. These are: human topoisomerase IIα [Sciandrello et al, 2010]; human-brain creatine kinase [Lü Z-R. et al, 2009]; human aldolase [Dobryszycki et al, 1999a;1999b]; human serum albumin [Ferguson et al, 2010]; human glyceraldehyde-3-phosphate dehydrogenase [Sakamoto & Hashimoto, 1985] and human enolase [Howland et. al., 1980].…”

Section: Proteinmentioning

confidence: 99%