The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat

Kornjaca, Dusko; Živković, Vladimir; Krstić, Danijela; Čolović, Mirjana B.; Djurić, M.; Stanković, Sanja; Mutavdzin, Slavica; Jakovljević, Vladimir; Djurić, Dragan

doi:10.2298/abs170731041k

Cited by 2 publications

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“…On the other hand, the same study indicates an increase in MDA in hippocampus after 3 h 40 , which may be the result of increased concentrations of iron, lipid content and low activity of antioxidants in basal conditions 41 . Other autors demonstrated that acutely given Met increased SOD and GPx activity 1h after application, but decreased CAT activity in the rat brain cortex 42 . In another experiment SOD activity in the rat brain remained unchanged, which is consistent with the results obtained in the heart in our study.…”

Section: Discussionmentioning

confidence: 99%

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

Mićović

Kostic

Mutavdzin

et al. 2020

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Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1?0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175043]

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Section: Discussionmentioning

confidence: 99%

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

Mićović

Kostic

Mutavdzin

et al. 2020

VSP

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The water extract of Rheum palmatum has antioxidative properties and inhibits ROS production in mice

Ma,

Yang,

Degen

et al. 2024

Journal of Ethnopharmacology

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The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat

Abstract: The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat.

Cited by 2 publications

References 32 publications

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue

The water extract of Rheum palmatum has antioxidative properties and inhibits ROS production in mice

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