The aim of this study is to research the effects of adhesive thickness, which is one of the factors that affect the stress distribution in the prismatic plug-in joints. The effect of adhesive thickness was theoretically researched in the study. Epoxy-based and acrylic-based adhesives, which are widely used in combining metal joints, were applied. After testing the mechanical characteristics of the adhesives, the models of the prismatic plug-in joints combined with adhesive were designed in the Pro-engineer program. The mechanical analysis of the models was performed in Ansys Workbench and the results are presented as a comparison.