The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury

Abstract

AIM:To investigate the effectiveness of rat adipose tissue-derived (rAT) mesenchymal stem cell (MSC) transplantation on the functional restoration and regeneration of spinal cord injury (SCI). MATERIAl and METhODS:Six of 48 Wistar albino rats were sacrificed to obtain MSCs, and the remaining rats were divided randomly into six groups. SCI was performed using the clip compression method. The control and transplantation groups were injected with physiological saline and a rAT-MSC suspension at the injury sites, respectively. Each animal was evaluated using the Basso, Beattie and Bresnahan (BBB) rating system and sacrificed at 28 days post-injury period (p.i.). The regeneration process was evaluated based on immunostaining against β3-tubulin, BDNF, CNTF, and CNPase.RESulTS: rAT-MSC transplantation into the SCI site substantially improved the tissue regeneration and functional recovery (p < 0.05). However, the rAT-MSC transplantation at 9 days p.i. was not more efficient on functional recovery than the transplantation immediately after injury. The expression of β3-tubulin, BDNF and CNTF at the injury site indicated the potential for functional regeneration. COnCluSIOn:The adaptive nature of rat-MSCs enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of SCI.

Neuronal regeneration in injured rat spinal cord after human dental pulp derived neural crest stem cell transplantation

“…Besides, the loss of function after SCI might be from both the primary mechanical insult and the subsequent, multifaceted secondary degenerative response. However, some experimental studies in last decades proved that the injured spinal cord could be restored (5)(6)(7)(8)(9). Nowadays, stem cell based therapy is promising some valuable strategies for functional recovery of the injured spinal cord (10).…”

Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

“…6a–c ). Moreover, CNTF has the ability to prevent scar tissue formation in the presence of other factors, such as BDNF [ 67 ]. This synergistic effect of CNTF with BDNF began to appear at 8 weeks after cell transplantation, resulting in the significantly higher stand/swing time ratio in the intermittent dASCs and sustaining dASCs 7d groups compared with the hollow and uASCs groups (Fig.…”

Prerequisites for Mesenchymal Stem Cell Transplantation in Spinal Cord Injury

“…Human mesenchymal cells from adipose tissue have deposited laminin and have promoted regeneration of injured spinal cord in rats [143][144][145][146]. Transplanted during the acute and subacute phases after spinal cord injury, they have enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of spinal cord injury [145]. Chondroitinase ABC-adipose-derived stem cells constructed using lentiviral vector transfection have stably expressed chondroitinase ABC, and chondroitinase ABC expression has significantly enhanced their migratory capacity [146].…”