The effects of amniotic membrane stem cell-conditioned medium on photoaging

Prakoeswa, Cita Rosita Sigit; Pratiwi, Febrina Dewi; Herwanto, Nanny; Citrashanty, Irmadita; Indramaya, Diah Mira; Murtiastutik, Dwi; Sukanto, Hari; Rantam, Fedik Abdul

doi:10.1080/09546634.2018.1530438

Cited by 43 publications

(37 citation statements)

References 18 publications

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“…In recent years, it has been found that adipose-derived MSCs have outstanding growth performance and differentiation potential [32], while MSCs derived from postpartum discarded tissues, such as the umbilical cord [4], placenta, and amniotic membrane are readily accessible with low cellular immunogenicity, and can be acquired from multiple sources. Therefore, MSCs have become a hot area of research [5,33]. Although a number of published reports on the biological characteristics of adipose-derived, umbilical cord, placenta, and amniotic membrane MSCs are available, the publications are mostly about human-derived MSCs [7,34].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

Zhan

El‐Ashram

Luo

et al. 2019

IJMS

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Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy. Comparing the biological and transcriptome gene characteristics of MSCs from different sources provides an important basis for the screening of clinically used cells. The main purpose of this experiment was to establish methods for the isolation and culture of MSCs from five different canine sources, including adipose tissue, bone marrow, umbilical cord, amniotic membrane, and placenta, and compare biological and transcriptome characteristics of MSCs, in order to provide a basis for the clinical application of canine MSCs. MSCs were isolated from Chinese pastoral dogs, and the following experiments were performed: (1) the third, sixth, and ninth generations of cells were counted, respectively, and a growth curve was plotted to calculate the MSC population doubling time; (2) the expression of CD34 and CD44 surface markers was studied by immunofluorescence; (3) the third generation of cells were used for osteogenetic and adipogenic differentiation experiments; and (4) MSC transcriptome profiles were performed using RNA sequencing. All of the five types of MSCs showed fibroblast-like adherent growth. The cell surface expressed CD44 instead of CD34; the third-generation MSCs had the highest proliferative activity. The average population doubling time of adipose mesenchymal stem cells (AD-MSCs), placenta mesenchymal stem cells (P-MSCs), bone marrow mesenchymal stem cells (BM-MSCs), umbilical cord mesenchymal stem cells (UC-MSCs), and amniotic mesenchymal stem cells (AM-MSCs) were 15.8 h, 21.2 h, 26.2 h, 35 h, and 41.9 h, respectively. All five types of MSCs could be induced to differentiate into adipocytes and osteoblasts in vitro, with lipid droplets appearing after 8 days and bone formation occurring 5 days after AD-MSC induction. However, the multilineage differentiation for the remaining of MSCs was longer compared to that of the AD-MSCs. The MSC transcriptome profiles showed that AD-MSC and BM-MSCs had the highest homology, while P-MSCs were significantly different compared to the other four types of MSCs. All the isolated MSCs had the main biological characteristics of MSCs. AD-MSCs had the shortest time for proliferation, adipogenesis, and osteogenic differentiation.

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Section: Discussionmentioning

confidence: 99%

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

Zhan

El‐Ashram

Luo

et al. 2019

IJMS

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“…It also contains cytokines such as interleukin (IL)-4 and IL-10 [ 14 ]. Prakoeswa has examined the effectiveness of AMSC metabolite products for photoaging with microneedling compared to placebo and obtained good results [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Amniotic Membrane Stem Cell (AMSC) Metabolite Product and Vitamin E for Wrinkles, Spots, and Pores in Photoaging

Rahmadewi

Retha

Pitasari

et al. 2020

Dermatology Research and Practice

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Background. It is expected that a combination of amniotic membrane stem cell metabolite product (AMSC-MP) and vitamin E after fractional CO2 laser as laser-assisted drug delivery (LADD) will provide better effects in photoaging treatment as the combination reaches the target. This promises an option for photoaging therapy in the future. Materials and Methods. Sixty women with photoaged skins were involved in this experimental study. They were then divided into two groups. The treatment group received a topical combination of AMSC-MP and vitamin E, and the control group received AMSC-MP alone after fractional CO2 laser. The treatment was repeated three times. Result. The Janus assessment results showed a significant difference in pores in the third observation, and the average pore improvements in the treatment group were better than the control group. Wrinkle, UV spot, and polar spot did not show any significant difference. Conclusion. A combination of the amniotic membrane stem cell metabolite product (AMSC-MP) and vitamin E after fractional CO2 laser as LADD only improves pores in photoaged skins.

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“…The use of these metabolite products are more accessible and safer because they are acellular; thus, easing the control and minimizing tissue rejection. [10][11][12] The synergistic effect of a combination of MP-AMSC and vitamin C is the accelerated wound healing process. Wound healing is a complex and dynamic process that involves various growth factors and cytokines.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolite Products Amniotic Membrane Stem Cell (MP-AMSC) derived from the amniotic membrane are used in skin rejuvenation to reduce wrinkles by stimulating fibroblast activation and collagen synthesis. [10][11][12] Vitamin C is a powerful antioxidant that can be used topically in dermatology settings to treat and prevent changes associated with photoaging. In a 2003 French study, ten women aged 51-59 years old were prescribed with a topical antioxidant, which was 5% vitamin C cream.…”

Section: Introductionmentioning

confidence: 99%

Long Term Effects of Combination of Metabolite Product Amniotic Membrane Stem Cell (MP-AMSC) and Vitamin C after Fractional CO2 Laser for Photoaging Treatment

Adiningtyas

Listiawan

Astari

2020

BIKK

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Background: Metabolite Products Amniotic Membrane Stem Cell (MP-AMSC) originating from the amniotic membrane has a skin rejuvenation effect by stimulating collagen synthesis. Vitamin C functions as an antioxidant in photoaging treatment. It is expected that the application of a combination of MP-AMSC and vitamin C after fractional CO2 laser treatment as Laser-Assisted Drug Delivery (LADD) to provide better long-term effects on clinical improvement of photoaged skin. As the drug penetration reaching the target, long-term effects and no side effects are expected. This promises as an option for photoaging therapy in the future. Purpose: This study aimed to evaluate the long-term effects of a combination of topical MP-AMSC mixtures and vitamin C after fractional CO2 laser treatment on wrinkles, spots, pores, and skin tones. Methods: Thirty adult women with photoaged skins were selected as samples, and were observed for wrinkles, spots, pores, and skin tones examination. The observation involved computer-simulated photographic devices, Facial Skin Scope System Janus-II for three months after the topical administration of MP-AMSC mixture and vitamin C, three times fractional CO2 laser, tretinoin 0.025% cream, and sun protection factor (SPF) 30 sunscreen as maintenance therapy. Result: The data analysis result was not statistically significant (p>0.05). Conclusion: There was a long-term effect of the application of a mixture of topical MP-AMSC and vitamin C after fractional CO2 laser treatment, and no side effect was observed.

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The effects of amniotic membrane stem cell-conditioned medium on photoaging

Cited by 43 publications

References 18 publications

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

A Comparative Study of Biological Characteristics and Transcriptome Profiles of Mesenchymal Stem Cells from Different Canine Tissues

The Efficacy of Amniotic Membrane Stem Cell (AMSC) Metabolite Product and Vitamin E for Wrinkles, Spots, and Pores in Photoaging

Long Term Effects of Combination of Metabolite Product Amniotic Membrane Stem Cell (MP-AMSC) and Vitamin C after Fractional CO2 Laser for Photoaging Treatment

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