The effects of antibiotics on the reproductive physiology targeting ovaries in the Asian tiger mosquito, <scp><i>Aedes albopictus</i></scp>

Ha, Young-Ran; Jeong, Se‐Jin; Jang, Chang-Won; Chang, Kyu‐Sik; Kim, Hyun‐Woo; Cho, Shin‐Hyoung; Lee, Hee‐Il

doi:10.1111/1748-5967.12462

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…An upset plot was created using the UpsetR package v1.4.0 (Conway et al, 2017) to visualize the number of DEGs in each pairwise comparison between recipients of a transplant and the conventionally reared control, as well as to identify DEGs that were common to every transplant recipient group. The ComplexHeatmap package v2.12.0 (Gu, 2022) was then used to visualize the log 2 fold changes of DEGs identified in each transplant recipient group relative to the conventionally reared control. Finally, a Gene Ontology (GO) enrichment analysis was performed using the VectorBase Gene Ontology Enrichment Analysis tool with default parameters to identify functions of commonly enhanced and suppressed DEGs (VectorBaseIDs), retaining those terms (within the ontology categories biological process, molecular function, and cellular component) that passed a threshold of Bonferroni adjusted p-value <0.05.…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally, manipulating the microbiome has involved treating mosquitoes with antibiotics that alter microbiome composition but can also affect mosquito physiology (Chabanol et al, 2020; Ha et al, 2021). However, approaches rearing axenic (germ‐free) mosquito larvae followed by supplementation with defined bacterial assemblages to produce gnotobiotic mosquitoes have since proven to be an excellent way to interrogate host–microbe interactions without using antibiotics, thus removing effects of the antibiotic and the ‘original’ microbiome.…”

Section: Introductionmentioning

confidence: 99%

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Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes

Hegde,

Brettell,

Quek

et al. 2024

Environmental Microbiology

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The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito species, life stages, and geography. How the host controls and is affected by this variation is unclear. Using microbiome transplant experiments, we asked whether there were differences in transcriptional responses when mosquitoes of different species were used as microbiome donors. We used microbiomes from four different donor species spanning the phylogenetic breadth of the Culicidae, collected either from the laboratory or the field. We found that when recipients received a microbiome from a donor reared in the laboratory, the response was remarkably similar regardless of donor species. However, when the donor had been collected from the field, many more genes were differentially expressed. We also found that while the transplant procedure did have some effect on the host transcriptome, this is likely to have had a limited effect on mosquito fitness. Overall, our results highlight the possibility that variation in mosquito microbiome communities is associated with variability in host–microbiome interactions and further demonstrate the utility of the microbiome transplantation technique for investigating host–microbe interactions in mosquitoes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes

Hegde,

Brettell,

Quek

et al. 2024

Environmental Microbiology

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show abstract

“…Traditionally, manipulating the microbiome has involved treating mosquitoes with antibiotics that alter microbiome composition, but can also affect mosquito physiology (Chabanol et al, 2020; Ha et al, 2021). However, approaches rearing axenic (germ-free) mosquito larvae followed by supplementation with bacteria of choice have proven to be an excellent way to interrogate host-microbe interactions without using antibiotics, thus removing effects of the antibiotic and the ‘original’ microbiome.…”

Section: Introductionmentioning

confidence: 99%

Aedes aegyptigut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes

Hegde

Brettell

Quek

et al. 2023

Preprint

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The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito species, life stages, and geography. How the host controls and is affected by this variation is unclear. Using microbiome transplant experiments, we asked whether there were differences in transcriptional responses when mosquitoes of different species were used as microbiome donors. We used microbiomes from four different donor species spanning the phylogenetic breadth of the Culicidae, collected either from the laboratory or field. We found that when recipients received a microbiome from a donor reared in the laboratory, the response was remarkably similar regardless of donor species. However, when the donor had been collected from the field, far more genes were differentially expressed. We also found that while the transplant procedure did have some effect on the host transcriptome, this is likely to have had a limited effect on mosquito fitness. Overall, our results highlight the possibility that variation in mosquito microbiome communities are associated with variability in host-microbiome interactions and further demonstrate the utility of the microbiome transplantation technique.

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“…Antibiotic exposure may largely affect the composition of the mosquito microbiota and, consequently, affect their lifespan and their competence for the transmission of vector-borne pathogens (e.g., Dong et al, 2009;Gendrin et al, 2015;Martínez-de la Puente et al, 2021). In addition, antibiotic treatments may also affect mosquito fecundity (Ha et al, 2021), blood digestion and egg production (Gaio et al, 2011), and the larval development period (Chouaia et al, 2012). Despite the importance of antibiotics in the epidemiology of mosquito-borne pathogens, studies so far have mainly focused on female mosquitoes belonging to the genera Anopheles (Dos Santos et al, 2022) and Aedes (Gómez-Govea et al, 2022), with very limited information available for other major mosquito vector groups.…”

Section: Introductionmentioning

confidence: 99%

Survival effects of antibiotic exposure during the larval and adult stages in the West Nile virus vector Culex pipiens

et al. 2023

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The ability of mosquitoes to transmit a pathogen is affected, among other factors, by their survival rate, which is partly modulated by their microbiota. Mosquito microbiota is acquired during the larval phase and modified during their development and adult feeding behavior, being highly dependent on environmental factors. Pharmaceutical residues including antibiotics are widespread pollutants potentially being present in mosquito breeding waters likely affecting their microbiota. Here, we used Culex pipiens mosquitoes to assess the impact of antibiotic exposure during the larval and adult stages on the survival rate of adult mosquitoes. Wild‐collected larvae were randomly assigned to two treatments: larvae maintained in water supplemented with antibiotics and control larvae. Emerged adults were subsequently assigned to each of two treatments, fed with sugar solution with antibiotics and fed only with sugar solution (controls). Larval exposure to antibiotics significantly increased the survival rate of adult females that received a control diet. In addition, the effect of adult exposure to antibiotics on the survival rate of both male and female mosquitoes depended on the number of days that larvae fed ad libitum in the laboratory before emergence. In particular, shorter larval ad libitum feeding periods reduced the survival rate of antibiotic‐treated adult mosquitoes compared with those that emerged after a longer larval feeding period. These differences were not found in control adult mosquitoes. Our results extend the current understanding of the impact of antibiotic exposure of mosquitoes on a key component of vectorial capacity, that is the vector survival rate.

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The effects of antibiotics on the reproductive physiology targeting ovaries in the Asian tiger mosquito, Aedes albopictus

Cited by 6 publications

References 42 publications

Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes

Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes

Aedes aegyptigut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes

Survival effects of antibiotic exposure during the larval and adult stages in the West Nile virus vector Culex pipiens

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