The effects of antimicrobials and lipopolysaccharide on acute immune responsivity in pubertal male and female CD1 mice

Esposito, Pasquale; Kearns, Madeleine May; Smith, Kevin B.; Chandrasegaram, Rajini; Kadamani, Anthony K.; Gandelman, Michelle; Liang, Jacky; Nikpoor, Naghmeh; Tompkins, Thomas A.; Ismail, Nafissa

doi:10.1016/j.cpnec.2022.100147

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…At five weeks of age, mice were administered 200 μL of mixed broad-spectrum antimicrobial solution or water through gavage twice daily for 7 day as described in ( Esposito et al, 2022 ). Briefly, the antimicrobial solution was made fresh daily and contained 15 mg/mL of ampicillin (No.…”

Section: Methodsmentioning

confidence: 99%

The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

Esposito

Gandelman

Rodriguez

et al. 2022

Brain, Behavior, & Immunity - Health

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Section: Methodsmentioning

confidence: 99%

The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

Esposito

Gandelman

Rodriguez

et al. 2022

Brain, Behavior, & Immunity - Health

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“…For example, exposure to Kefir containing active bacterial culture ( L. lactis, L. cremoris, L. diacetylactis, L. acid-ophilus ), lactic yeasts and skim milk powder one-week prior to and one-week following pubertal LPS treatment reduced sickness behavior ( Murray et al, 2019 ). Another study using a probiotic mixture containing Lactobacillus helveticus and Bifidobacterium longum showed decreased cytokine concentrations in the blood of CD-1 mice ( Esposito et al, 2022 ). These findings hold significant implications, as elevated levels of inflammatory cytokines are known to be associated with an increased risk of neuropsychiatric disorders and depression ( Felger and Lotrich, 2013 ).…”

Section: Link Between the Gut Microbiome And Mental Healthmentioning

confidence: 99%

Enduring sex-dependent implications of pubertal stress on the gut-brain axis and mental health

Dworsky-Fried,

Tchida,

Krnel

et al. 2024

Front. Behav. Neurosci.

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The gut-brain axis (GBA) is a network responsible for the bidirectional communication between the central nervous system and the gastrointestinal tract. This multifaceted system is comprised of a complex microbiota, which may be altered by both intrinsic and extrinsic factors. During critical periods of development, these intrinsic and extrinsic factors can cause long-lasting sex-dependent changes in the GBA, which can affect brain structure and function. However, there is limited understanding of how the GBA is altered by stress and how it may be linked to the onset of mental illness during puberty. This article reviews current literature on the relationships between the GBA, the effects of stress during puberty, and the implications for mental health.

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“…Selecting outbred mice increases genetic diversity and offers greater external validity to our experimental findings (Voelkl et al., 2020). These mice elicit acute and enduring negative changes in response to pubertal stress (Esposito, Gandelman et al., 2022; Esposito, Kearns et al., 2022; Murack et al., 2021, 2023; Murray et al., 2019, 2020; Smith, Murray, Chandrasegaram et al., 2021; Smith, Murray, Gregory et al., 2021). All experiments must receive ethics approval from institutional animal care and ethics committee before proceeding.…”

Section: Commentarymentioning

confidence: 99%

Environmental Enrichment Cage for Laboratory Mice: A Downloadable Alternative

Smith,

Murack,

Sharani

et al. 2024

Current Protocols

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Environmentally enriched housing (EE) provides a stimulating and species‐typical environment that enhances brain plasticity and cognition, while reducing disease severity in laboratory animals. However, standardizing EE protocols has been challenging due to issues such as variability, high pricing, or limited laboratory space. To address these challenges, we present a replicable and cost‐efficient cage protocol that is accessible to researchers with limited resources and space constraints. The protocol is designed to provide a stimulating and species‐typical environment for the animals. It incorporates elements such as social interaction, physical exercise, cognitive stimulation, manipulable objects, environmental variability, and sensory stimulation. As evidenced in our previous studies using our protocol, users can expect to observe similar neuroplastic and health‐wise benefits that accompany EE experimental paradigms. These included straightforward step‐by‐step guide, which allows for construction of functional EE cages in under 8 hr. Basic knowledge of 3D printing and laser cutting is required, but no advanced skills are necessary. The protocol enables researchers to create stimulating and replicable environments that promote animal welfare, enhance brain plasticity, and yield valuable experimental results for low cost. © 2024 Wiley Periodicals LLC.Basic Protocol: An effective and cost‐efficient environmental enrichment cage designed to encourage standardization amongst laboratory protocols.

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The effects of antimicrobials and lipopolysaccharide on acute immune responsivity in pubertal male and female CD1 mice

Cited by 5 publications

References 47 publications

The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

Enduring sex-dependent implications of pubertal stress on the gut-brain axis and mental health

Environmental Enrichment Cage for Laboratory Mice: A Downloadable Alternative

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