The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs

Jin, Hongting; Xia, Bingjiang; Yu, Nanze; He, Bo; Shen, Yan; Xiao, Lan; Tong, Peijian

doi:10.1007/s00264-012-1674-7

Cited by 32 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6,9 There is an inverse relationship between osteogenesis and adipogenesis; typically, increased osteogenesis is associated with decreased adipogenesis. [23][24][25] Furthermore, quite a number studies have manifested that transplanted autologous or allogeneic MSCs were capable of survival, proliferation, and differentiation into osteoblasts directly in the necrotic femoral head on humans 26-28 and a variety of animal models; for example, in mouse, 29 rabbit, 30,31 dog, 32 and sheep. 33 Thus, regulation on the osteogenesis and adipogenesis of MSCs is the novel therapeutics for ONFH treatment.…”

Section: Discussionmentioning

confidence: 99%

Aqueous Fraction of Huogu Formula Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells Through the BMP and Wnt Signaling Pathways

Kong

Zhang

et al. 2016

Rejuvenation Research

View full text Add to dashboard Cite

Our recent studies have shown that Huogu (HG) formula was effective both in clinic experience and in experimental osteonecrosis of the femoral head (ONFH). Given that defective of bone marrow stromal cells (MSCs) contribute to the development of osteonecrosis and MSCs show enormous potential in the treatment of ONFH, especially to aging people. How HG impacts the differentiation of MSCs and what is the underlying cellular and molecular mechanism remains largely unknown. Here, we found that an aqueous fraction of HG (HGA) significantly increased the alkaline phosphatase (ALP) activity, mineralized nodules, and migration of MSCs in a dose-dependent manner. Meanwhile, HGA could enhance the mRNA and protein expression of Runt-related transcription factor 2 (Runx2), Alp, Bmp2, osteocalcin (Ocn), and Osterix (Osx). Further investigation of the molecular mechanisms revealed that HGA treatment obviously increased expression, secretion, and activation of bone morphogenetic protein (BMP) 2 and b-catenin, two key regulators of the BMP or Wnt signaling pathway. Furthermore, osteogenic differentiation of MSCs could be blocked by using pharmacological inhibitors for these signaling pathways such as Noggin and Dkk-1. Besides, HGA could inhibit adipogenic differentiation of MSCs. Our study reveals that HGA promotes the osteogenesis of MSCs via the BMP and Wnt signaling pathways. Our findings provide mechanistic insights into the role of HG in treating ONFH.

show abstract

Section: Discussionmentioning

confidence: 99%

Aqueous Fraction of Huogu Formula Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells Through the BMP and Wnt Signaling Pathways

Kong

Zhang

et al. 2016

Rejuvenation Research

View full text Add to dashboard Cite

show abstract

“…It has been proved that most hip-preserving surgery can achieve good outcomes only when the necrotic areas are limited to small parts of the femoral head [29]. Current available treatments focusing on reconstruction of the blood supply of femoral head can frequently largely improve the curative effect [30][31][32][33][34]. So does vascularized greater trochanter flap grafting.…”

Section: Discussionmentioning

confidence: 99%

Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head

Zeng

He²,

Feng³

et al. 2013

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose To investigate the curative efficacy of osteonecrosis of the femoral head (ONFH) in a hip-preserving operative approach, by grafting a vascularized greater trochanter flap combined with a free iliac flap, in an attempt to seek an innovative approach for patients who suffered middle to late stage ONFH without total hip arthroplasty (THA) surgery. Method Our research included a total of 60 patients (66 hips) who accepted hip-preserving surgery by grafting a vascularized greater trochanter flap combined with a free iliac flap which was tightly filled by hammering because of ONFH (most were Association Research Circulation Osseous (ARCO) stage III patients) from January, 2006 to December, 2010. A Harris Hip Score was obtained during follow-ups, evaluating the clinical efficacy, X-rays were taken regularly for image assessing, and the SF-36 scale was used for estimating quality of life. Terminal observation time was considered when patients had symptom-dependant indications for performing another hip-preserving surgery or THA surgery. Results Fifty-eight patients (64 hips) were eventually contacted by telephone for an out-patient clinic return visit, with a mean follow-up time of 35.8 months (varied from 12 months to 60 months), but two patients lost contact for various reasons. The demographic data were as follows: there were 16 ARCO IIIA cases, 22 ARCO IIIB cases, and 26 ARCO IIIC cases, respectively. Postoperative X-rays revealed a well-repaired necrotic area of the femoral head and improvement of femoral-acetabulum coverage. The last follow-up mean Harris Hip Score was 86.56±7.38 (excellent results reached 87.50 %), which were greatly improved compared to 50.95±6.86 pre-operatively. Also the postoperative mean scores of all dimensions of the SF-36 scale were improved to some extent. Additionally the physical component summary (PSC) scores were enhanced from 42± 13 pre-operatively to 78±11, while the postoperative mental component summary (MCS) scores (76±11) largely increased in contrast to pre-operative scores (51±10), with both target indices having statistical significance (p=0.005, p=0.01), signifying hugely improvement of the quality of life of the patients. A correlation was found between Harris Hip Score and all dimensions of SF-36 scale (r=0.32-0.72), especially closely correlated with physical functioning (PF), role-physical (RP) and bodily pain (BP) in PCS aspect (r= 0.72, p < 0.01; r = 0.58, p < 0.01; r = 0.65, p < 0.01, respectively). Conclusion There is definite curative efficacy for the treatment of ONFH with an hip-preserving operative approach by grafting a vascularized greater trochanter flap combined with a free iliac flap which was tightly filled by hammering. This kind of operative approach reconstructs the biological stability of femoral head, which promotes repair of necrotic areas and indirectly preserves the femoral head of patients and a majority of hip function. It possesses vast clinical as well as practical significance, because the long-term efficacy can satisfy fundam...

show abstract

“…However, a variety of joint-preserving operative treatment methods have been introduced because ONFH occurs predominantly in the young, and these treatment options produce promising results when the procedure is implemented before joint deterioration [4][5][6]. The advent of magnetic resonance imaging (MRI) has facilitated the diagnosis of asymptomatic ONFH; however, the natural history of asymptomatic ONFH is not yet understood.…”

Section: Introductionmentioning

confidence: 99%

“…The advent of magnetic resonance imaging (MRI) has facilitated the diagnosis of asymptomatic ONFH; however, the natural history of asymptomatic ONFH is not yet understood. Furthermore, guidelines for the treatment of this condition have not been well defined [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

The natural history of asymptomatic osteonecrosis of the femoral head

Kang

Moon

Kwon

et al. 2013

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose To observe the natural history of asymptomatic osteonecrosis of the femoral head, and to analyse the associations between the subsequent development of symptoms, epidemiological risk factors and the character of the lesions. Methods Sixty-eight patients were diagnosed with asymptomatic osteonecrosis of the femoral head. The patients were classified based on the development of symptoms. Relations were sought between symptom development and epidemiological risk factors, and the size and location of the necrotic lesions. Results Thirty-eight patients developed symptoms (55.9 %) at a mean 2.27 years after diagnosis. Symptoms developed in 18 of 28 patients with alcohol-related necrosis (64.3 %), in eight of 14 patients with steroid-related necrosis (57.1 %), and in 12 of 26 patients with idiopathic necrosis (46.2 %). None of the following: gender, age, body mass index (BMI), smoking status, or cholesterol level, was found to be significantly associated with the development of symtoms in asymptomatic osteonecrosis of femoral head (ONFH). Duration and amount of exposure to steroid were not significantly associated with symptom development. In the groups of heavy alcohol drinkers, large necrotic lesions and laterally located lesions showed a higher prevalence of symptom development. Conclusion Symptoms developed in 55.9 % of asymptomatic osteonecrosis of the femoral head. Prevalence of symptom development was significantly higher in heavy alcohol drinkers and large-sized lateral lesions.

show abstract

The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs

Cited by 32 publications

References 26 publications

Aqueous Fraction of Huogu Formula Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells Through the BMP and Wnt Signaling Pathways

Aqueous Fraction of Huogu Formula Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells Through the BMP and Wnt Signaling Pathways

Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head

The natural history of asymptomatic osteonecrosis of the femoral head

Contact Info

Product

Resources

About