The Effects of Baicalin Against UVA-Induced Photoaging in Skin Fibroblasts

Abstract

Ultraviolet A (UVA) radiation contributes to skin photoaging. Baicalin, a plant-derived flavonoid, effectively absorbs UV rays and has been shown to have anti-oxidant and anti-inflammatory properties that may delay the photoaging process. In the current study, cultured human skin fibroblasts were incubated with 50 μg/ml baicalin 24 hours prior to 10 J/cm(2) UVA irradiation. In order to examine the efficacy of baicalin treatment in delaying UVA-induced photoaging, we investigated aging-related markers, cell cycle changes, anti-oxidant activity, telomere length, and DNA damage markers. UVA radiation caused an increased proportion of β-Gal positive cells and reduced telomere length in human skin fibroblasts. In addition, UVA radiation inhibited TGF-β1 secretion, induced G1 phase arrest, reduced SOD and GSH-Px levels, increased MDA levels, enhanced the expression of MMP-1, TIMP-1, p66, p53, and p16 mRNA, reduced c-myc mRNA expression, elevated p53 and p16 protein expression, and reduced c-myc protein expression. Baicalin treatment effectively protected human fibroblasts from these UVA radiation-induced aging responses, suggesting that the underlying mechanism involves the inhibition of oxidative damage and regulation of the expression of senescence-related genes, including those encoding for p53, p66(Shc) and p16.

Human amnion-derived mesenchymal stem cells protect against UVA irradiation-induced human dermal fibroblast senescence, in vitro

“…Human amnion-derived mesenchymal stem cells were prepared as described previously ( 3 ). Briefly, human amniotic membrane was mechanically peeled off from the chorion of a placenta obtained from an uncomplicated elective caesarean section with the informed consent of the donor patient.…”

“…UVA radiation caused an increased proportion of β-Gal positive cells and reduced telomere length in human skin fibroblasts. In addition, UVA radiation inhibited TGF-β 1 secretion, induced G1 phase arrest, reduced SOD and GSH-Px levels, increased MDA levels and the expression of MMP-1, TIMP-1, p66, p53 mRNA expression ( 2 , 3 ).…”

“…Human amnion-derived mesenchymal stem cells (HAMSCs) obtained from human amniotic membrane (AM) are readily available and high abundant tissue, with substantial benefits as seed cells ( 3 , 4 ). Their low anti-flammatory properties and fewer ethical concerns compared with other sources of stem cell are clear advantage ( 5 – 7 ).…”

Baicalin Inhibits the Fenton Reaction by Enhancing Electron Transfer from Fe2+ to Dissolved Oxygen

“…Several studies have verified the anti-inflammatory effects of baicalin, demonstrating that baicalin inhibits carrageenan-induced paw edema in rats, protects against ischemia-reperfusion injury in cardiomyocytes and brain tissue, prevents cerebrovascular dysfunction in experimental heat stroke in rats, and has therapeutic effects on lipopolysaccharide-induced acute lung injury in rats (Lin and Shieh, 1996;Zhang et al, 2006b;Chang et al, 2007a,b;Huang et al, 2008). Furthermore, baicalin has been shown to have anti-oxidant properties and may therefore be effective in some clinical conditions, such as inflammatory diseases, disorders affecting the immune system, and iron overload, in which reactive species are thought to be involved (Gao et al, 1999;Halliwell and Gutteridge, 2007;Min et al, 2014). Indeed, baicalin has been shown to scavenge free radicals in human leukocytes (Shen et al, 2003) and the rat brain (Hamada et al, 1993), and has a strong affinity for iron ions (Perez et al, 2009).…”

UV-Induced Melanoma Mouse Model Dependent on Endothelin 3 Over-Expression

“…The UVB rays penetrate superficially into the skin and have higher absorption rate by DNA than UVA, as well as being considered more mutagenic and cytotoxic than UVA (Cadet et al, 1997;Jhappan et al, 2003). Additionally, a UVA dose has to be 1000 times stronger than that of UVB to produce the same level of erythema (Parrish et al, 1982 (Burke, 2010;Fisher et al, 2002;Ichihashi et al, 2003;Mason and Reichrath, 2013;Min et al, 2014;Ullrich, 2002;Yamaguchi et al, 2008).…”