The effects of bile salt and raw bile on the intestinal absorption of micellar fatty acid in the rat <i>in vitro</i>

Rampone, Alfred J.

doi:10.1113/jphysiol.1972.sp009821

Cited by 13 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible that lecithin reacts with the epithelial membrane itself, reducing its permeability. This appears unlikely since it was shown in a previous study that whole bile did not alter the uptake of a glucose analogue, 3-0-methyl-D-glucose (Rampone, 1972a ALFRED J. RAMPONE A more likely possibility is that the presence of lecithin in mixed micelles results in the production of micelles of expanded size. This in turn would reduce the permeability of either the contained cholesterol or of the intact micelles (if micelles are taken up intact).…”

Section: Possible Mechanism Ofaction Of Lecithinmentioning

confidence: 86%

“…It is likely that it would also have a bearing on the absorption of some of the other component parts of mixed micelles. For example, it has been shown that whole bile suppresses intestinal oleic acid uptake from micelles and this response may take place by a mechanism similar to that influencing cholesterol absorption (Rampone, 1972a(Rampone, , 1973.…”

Section: Discussionmentioning

confidence: 99%

“…Other experiments were done to rule out a non-specific or 'toxic' effect of whole bile such as might occur, for example, from the deconjugation of bile salts during storage (Rampone, 1972a). Based on these and other studies (Isaksson, 1954;Thureborn, 1963;Nakayama & Miyaki, 1965;Neiderhiser, Roth & Webster, 1966;Bourges, Small & Dervichian, 1967;Rampone, 1972b), it was suggested that the non-bile salt component may be lecithin.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of lecithin on intestinal cholesterol uptake by rat intestine in vitro

Rampone¹

1973

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

SUMMARY1. Sacs 20 cm long were obtained from the upper half of the small intestine of bile fistula rats (bile duct cannulated 48 hours previously). The sacs were everted, filled with oxygenated phosphate buffer and incubated 1 hr at 37"Cin 25 ml. of a buffered micellar solution of oleic acid (0.6 mM), mono-olein (0.3 mM), sodium taurocholate (4.8 mM) and 3H-labelled cholesterol (0-15 mM) plus glucose (28 mM 3. Adding 3 ml. whole rate bile with other factors unchanged caused cholesterol uptake to decrease by 50 % in confirmation of previous studies.4. Adding purified lecithin obtained from rat liver tissue, and from egg yolks, similarly decreased cholesterol uptake. A significant response was obtained with 2-5 mg liver lecithin (concentration 0-13 mM) and a near maximum response with 15 mg (concentration 0-80 mM). 10 mg lecithin decreased uptake by an amount equivalent to that obtained with 3 ml. whole bile.5. Lecithin is an active component of whole bile causing reduced intestinal cholesterol uptake from micelles.6. The decreased uptake of cholesterol in the presence of lecithin may have been the result of expansion of the cholesterol-containing micelles with consequent reduction in cholesterol permeability.

show abstract

Section: Possible Mechanism Ofaction Of Lecithinmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of lecithin on intestinal cholesterol uptake by rat intestine in vitro

Rampone¹

1973

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The lipid was then subjected to thin layer chromatography on silica gel plates and the incorporation of the 14C-labelled oleic acid into the free fatty acid and triglyceride fractions was measured by liquid scintillation spectrometry (Brown & Johnston, 1962). See previous publication for detailed description (Rampone, 1972a). 14C incorporation into other lipid fractions was not measured because, as shown previously (Rampone, 1972 a), approximately 90 % of the recovered label was accounted for in the sum of the free fatty acid and triglyceride fractions.…”

Section: Measurement Of Fatty Acid Uptakementioning

confidence: 99%

“…However, in a recent report (Rampone, 1972a) it was demonstrated that there are other components in bile which may be important in determining fatty acid absorption from micelles. Sacs of everted rat intestine were incubated for one hour in a solution of bile salt, monoglyceride and 14C-labelled fatty acid in micellar form and the tissue uptake of the label and its incorporation into triglyceride were measured.…”

Section: Introductionmentioning

confidence: 99%

Studies on micellar fatty acid uptake by rat intestine in vitro with reference to the role of bile

Rampone¹

1973

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

1. The uptake and esterification to trigylceride of oleic acid in micellar form was studied in rat intestine in vitro. Sacs of the upper half of the everted intestine taken from bile fistula rats were incubated in a buffered solution containing mono‐olein, 14C‐labelled oleic acid and bile salt (sodium taurocholate (NaTch) in concentrations exceeding the critical micellar concentration). 2. At 37° C incubation temperature increasing the NaTch concentration enhanced both uptake and esterification. Adding whole rat bile caused uptake to decrease at all NaTch concentrations but had only a slight and variable effect on esterification. 3. Lowering the incubation temperature to 0° C suppressed esterification but had no effect on uptake. 4. At 0° incubation temperature adding whole bile still decreased fatty acid uptake but had no effect on esterification. 5. It is concluded that intestinal fatty acid uptake from micelles is a non‐energy requiring process and that a non‐bile salt component in bile exists which can suppress this process. 6. It is suggested that lecithin may be the non‐bile salt component and that it suppressed uptake by interacting either with the micelles or with the epithelial membrane to reduce fatty acid permeability.

show abstract

Impaired jejunal transport of monosaccharides in experimental cholestasis

et al. 1973

View full text Add to dashboard Cite

The effects of bile salt and raw bile on the intestinal absorption of micellar fatty acid in the rat in vitro

Abstract: SUMMARY1. The uptake, esterification and transport of [l4C]oleic acid were studied using sacs of rat everted small intestine incubated in 25 ml. of a buffered mixture of sodium taurocholate, glyceryl mono-oleate and 14C-labelled oleic acid in micellar form.

Cited by 13 publications

References 17 publications

The effect of lecithin on intestinal cholesterol uptake by rat intestine in vitro

The effect of lecithin on intestinal cholesterol uptake by rat intestine in vitro

Studies on micellar fatty acid uptake by rat intestine in vitro with reference to the role of bile

Impaired jejunal transport of monosaccharides in experimental cholestasis

Contact Info

Product

Resources

About