The Effects of Biosynthesis Silver Nanoparticle on Oxidative Stress Parameters in Model Organism Artemia salina (Linnaeus, 1758)

Abstract

As a result of the release of nanomaterials into the ecosystem, the possibility of living things being exposed to these nanostructures has also increased. For this reason, nanotoxicology studies have gained importance. Evaluation of the effects of nanomaterials in various habitats and living groups is increasing with in vitro and in vivo studies. This study aims to describe a biological procedure to synthesize silver nanoparticles using an extract of Marrubium astracanicum and to determine the aquatic environment's oxidative stress on Artemia salina. Silver nanoparticle formation was visually observed with color change. Its structural properties, such as size, shape, morphology, and stability, were characterized by UV-Vis spectrophotometer, TEM, SEM, XRD, and DLS analyses. The effects of biosynthesized silver nanoparticles at different concentrations(control,0.2, 1, 5, 10, 25, and 50 mg/mL) and exposure times (24, 48 and 72 hours) on total glutathione and lipid peroxidation levels were investigated on the Artemia salina organism, which is one of the most common saltwater organisms frequently used in ecotoxicity tests.