The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro

Keleş, İhsan; Sharma, Alok; Woldehiwet, Z.; Murray, Richard D.

doi:10.1016/s0147-9571(98)00023-x

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Suppression of in vitro T-cell responses by human RSV (43,46) and bovine RSV has been described (26,51,52). Unlike measles, however, it is not immediately obvious that suppression of T-cell responses in vitro is clinically relevant.…”

mentioning

confidence: 99%

Alpha and Lambda Interferon Together Mediate Suppression of CD4 T Cells Induced by Respiratory Syncytial Virus

Chi

Dickensheets

Spann

et al. 2006

J Virol

105

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mentioning

confidence: 99%

Alpha and Lambda Interferon Together Mediate Suppression of CD4 T Cells Induced by Respiratory Syncytial Virus

Chi

Dickensheets

Spann

et al. 2006

J Virol

105

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show abstract

“…In vitro, RSV infection of peripheral blood mononuclear cells (PBMCs) was reported to result in suppression or delay of proliferation of PBMCs after stimulation with phytohemagglutinin (PHA) or with Epstein-Barr virus antigen. Production of cytokines such as IFN-␣ and IL-1 inhibitor was considered responsible for the observed inhibition of PBMC proliferation (18,30,32).…”

mentioning

confidence: 99%

Respiratory Syncytial Virus Fusion Protein Mediates Inhibition of Mitogen-Induced T-Cell Proliferation by Contact

Schlender

Walliser

Fricke

et al. 2002

J Virol

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Human respiratory syncytial virus (HRSV) and bovine respiratory syncytial virus (BRSV) are major pathogens in infants and calves, respectively. Experimental BRSV infection of calves and lambs is associated with lymphopenia and a reduction in responsiveness of peripheral blood lymphocytes (PBLs) to mitogens ex vivo. In this report, we show that in vitro mitogen-induced proliferation of PBLs is inhibited after contact with RSV-infected and UV-inactivated cells or with cells expressing RSV envelope proteins on the cell surface. The protein responsible was identified as the RSV fusion protein (F), as cells infected with a recombinant RSV expressing F as the single envelope protein or cells transfected with a plasmid encoding F were able to induce this effect. Thus, direct contact with RSV F is necessary and sufficient to inhibit proliferation of PBLs. Interestingly, F derived from HRSV was more efficient in inhibiting human PBL proliferation, while F from BRSV was more efficient in inhibiting bovine PBLs. Since various T-cell activation markers were upregulated after presenter cell contact, T lymphocytes are viable and may still be activated by mitogen. However, a significant fraction of PBLs were delayed or defective in G 0 /G 1 to S-phase transit.

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RSV-induced prostaglandin E2 production occurs via cPLA2 activation: Role in viral replication

et al. 2005

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Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production.

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The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro

Cited by 4 publications

References 24 publications

Alpha and Lambda Interferon Together Mediate Suppression of CD4 T Cells Induced by Respiratory Syncytial Virus

Alpha and Lambda Interferon Together Mediate Suppression of CD4 T Cells Induced by Respiratory Syncytial Virus

Respiratory Syncytial Virus Fusion Protein Mediates Inhibition of Mitogen-Induced T-Cell Proliferation by Contact

RSV-induced prostaglandin E2 production occurs via cPLA2 activation: Role in viral replication

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