The effects of bupivacaine combined with different adjuvants on block onset and duration and on ion channel expressions (SCN9A, TRPM) in sciatic nerve block in rats

Dolu, Nurgül; Şahin, Levent; Kaplan, Davut Sinan; Demi̇r, Tuncer; Şimşek, Hasan; Şahin, Mehrican; Cengiz, Beyhan

doi:10.3906/sag-1502-4

Cited by 2 publications

(5 citation statements)

References 44 publications

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“…TRPM2 and TRPV1 expression levels are high in the DRGNs and overload Ca 2+ entry through the channels involved in neuropathic pain (Isami et al, 2013; Pecze et al, 2013; Akpınar et al, 2016) and apoptotic (Hara et al, 2002) processes. The association between overload Ca 2+ entry through TRPM2 and TRPV1 and peripheral pain intensity has been reported in sciatic nerve injury (SNI)-induced rats (Dolu et al, 2016). Accumulating evidence indicated that importance of oxidative stress, the TRPM2 and TRPV1 channels in DRGN and sciatic nerve injuries has been increasing in experimental animal and human (Facer et al, 2007; Frederick et al, 2007; Haraguchi et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

Inhibition of the TRPM2 and TRPV1 Channels through Hypericum perforatum in Sciatic Nerve Injury-induced Rats Demonstrates their Key Role in Apoptosis and Mitochondrial Oxidative Stress of Sciatic Nerve and Dorsal Root Ganglion

Nazıroğlu

Çiğ

2017

Front. Physiol.

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Sciatic nerve injury (SNI) results in neuropathic pain, which is characterized by the excessive Ca2+ entry, reactive oxygen species (ROS) and apoptosis processes although involvement of antioxidant Hypericum perforatum (HP) through TRPM2 and TRPV1 activation has not been clarified on the processes in SNI-induced rat, yet. We investigated the protective property of HP on the processes in the sciatic nerve and dorsal root ganglion neuron (DRGN) of SNI-induced rats. The rats were divided into five groups as control, sham, sham+HP, SNI, and SNI+HP. The HP groups received 30 mg/kg HP for 4 weeks after SNI induction. TRPM2 and TRPV1 channels were activated in the neurons by ADP-ribose or cumene peroxide and capsaicin, respectively. The SNI-induced TRPM2 and TRPV1 currents and intracellular free Ca2+ and ROS concentrations were reduced by HP, N-(p-amylcinnamoyl) anthranilic acid (ACA), and capsazepine (CapZ). SNI-induced increase in apoptosis and mitochondrial depolarization in sciatic nerve and DRGN of SNI group were decreased by HP, ACA, and CapZ treatments. PARP-1, caspase 3 and 9 expressions in the sciatic nerve, DRGN, skin, and musculus piriformis of SNI group were also attenuated by HP treatment. In conclusion, increase of mitochondrial ROS, apoptosis, and Ca2+ entry through inhibition of TRPM2 and TRPV1 in the sciatic nerve and DRGN neurons were decreased by HP treatment. The results may be relevant to the etiology and treatment of SNI by HP.

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Section: Introductionmentioning

confidence: 97%

Inhibition of the TRPM2 and TRPV1 Channels through Hypericum perforatum in Sciatic Nerve Injury-induced Rats Demonstrates their Key Role in Apoptosis and Mitochondrial Oxidative Stress of Sciatic Nerve and Dorsal Root Ganglion

Nazıroğlu

Çiğ

2017

Front. Physiol.

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“…For example, similar experimental studies have been conducted with dexamethasone, dexmedetomidine, epinephrine and many types of opioids -also easily accessible by anesthesiologists. These agents have found their place as adjuvant agents in clinical practice [15,[20][21][22]. By all means, the effect of combining sugammadex and bupivacaine on tissues including but not limited to myotoxicity and neurotoxicity require investigation through histopathological, toxicological and pharmacokinetic studies.…”

Section: Discussionmentioning

confidence: 99%

“…For this study, the preservative-free forms of the drugs were not available; therefore, commercially available forms were used. In accordance with a similar study, 0.2 ml was used as the volume for each sciatic block [ 15 ]. For all experimental groups except Group S and the sham group, 0.16 ml bupivacaine 0.5% was administered.…”

Section: Methodsmentioning

confidence: 99%

“…When lateral movement occurs, a fast but weak hopping reflex is observed if the motor blockade is strong. If the proprioceptive blockade is stronger, then the hopping response is delayed and increased lateral movement is required to elicit a response [ 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…The preservative-free forms of the drugs were not available, therefore market forms were used. 0.2 ml was chosen as the volume for each sciatic block, in accordance with a similar study [15]. 0.16 mL bupivacaine %0.…”

Section: Doses and Groupsmentioning

confidence: 99%

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Evaluation of the effects of bupivacaine combined with sugammadex on the duration of the nociceptive blockade in sciatic nerve blocks: a controlled, double-blind animal study

Taşargöl

Darbaz

Ergene

et al. 2023

Korean J Anesthesiol

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Background: Animal and other experimental studies have demonstrated increased block time and quality when alpha and beta cyclodextrin drugs are combined with the local anesthetic. However, to our knowledge there exists no study that has utilized gamma cyclodextrins in such a combination. In this present study we used an animal model to evaluate the effect of different doses of combined administration of gamma cyclodextrin (sugammadex) and bupivacaine on sciatic nerve blockage times in rats.Methods: Sciatic block was performed with 0.20 mL mixture in all groups. This mixture consisted of 0.2ml saline in the Sham group, 0.2ml sugammadex in Group S and 0.16ml bupivacaine 0.5% and 0.04ml saline in Group B. In the experimental groups, in addition to 0.16ml bupivacaine 0.5%, 0.01 ml sugammadex and 0.03ml saline was added in Group BS1 0.02ml sugammadex and 0.02ml saline was added in Group BS2 and 0.04ml sugammadex was added in Group BS4. Proprioception, nociception and motor function was evaluated until sciatic block was completely reversed.Results: Motor, proprioceptive and nociceptive block occurred in all experimental groups within 5 minutes. In Group BS4 the duration of motor, proprioceptive and nociceptive blocks significantly increased compared with other experimental groups. However in Group BS1 and Group BS2, only the duration of nociceptive block significantly increased. Conclusion:Combined administration of sugammadex and bupivacaine when performing sciatic nerve block in rats leads to a significant prolongation of motor, proprioceptive and nociceptive block times.

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The effects of bupivacaine combined with different adjuvants on block onset and duration and on ion channel expressions (SCN9A, TRPM) in sciatic nerve block in rats

Cited by 2 publications

References 44 publications

Inhibition of the TRPM2 and TRPV1 Channels through Hypericum perforatum in Sciatic Nerve Injury-induced Rats Demonstrates their Key Role in Apoptosis and Mitochondrial Oxidative Stress of Sciatic Nerve and Dorsal Root Ganglion

Inhibition of the TRPM2 and TRPV1 Channels through Hypericum perforatum in Sciatic Nerve Injury-induced Rats Demonstrates their Key Role in Apoptosis and Mitochondrial Oxidative Stress of Sciatic Nerve and Dorsal Root Ganglion

Evaluation of the effects of bupivacaine combined with sugammadex on the duration of the nociceptive blockade in sciatic nerve blocks: a controlled, double-blind animal study

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