The effects of cannabidiol on the antigen-induced contraction of airways smooth muscle in the guinea-pig

Dudášová, A.; Keir, Sandra; Parsons, M. E.; Molleman, Areles; Page, Clive

doi:10.1016/j.pupt.2013.02.002

Cited by 19 publications

(15 citation statements)

References 31 publications

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“…This suggests that the effects of CBD on lung mechanics and gas exchange were not only due to modulation of the inflammatory condition after HI but also due to other yet-to-be determined mechanisms. It has been reported that CBD has bronchodilator effects in guinea pigs exposed to exogenous spasmogens (30), but there is no information supporting such an effect in other conditions.…”

Section: Discussionmentioning

confidence: 97%

Cannabidiol reduces lung injury induced by hypoxic–ischemic brain damage in newborn piglets

et al. 2017

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BackgroundBrain hypoxic-ischemic (HI) damage induces distant inflammatory lung damage in newborn pigs. We aimed to investigate the effects of cannabidiol (CBD) on lung damage in this scenario.MethodsNewborn piglets received intravenous vehicle, CBD, or CBD+WAY100635 (5-HT receptor antagonist) after HI brain damage (carotid flow interruption and FiO 0.10 for 30 min). Total lung compliance (TLC), oxygenation index (OI), and extravascular lung water content (EVLW) were monitored for 6 h. Histological damage, interleukin (IL)-1β concentration, and oxidative stress were assessed in brain and lung tissue. Total protein content was determined in bronchoalveolar lavage fluid (BALF).ResultsCBD prevented HI-induced deleterious effects on TLC and OI and reduced lung histological damage, modulating inflammation (decreased leukocyte infiltration and IL-1 concentration) and reducing protein content in BALF and EVLW. These effects were related to CBD-induced anti-inflammatory changes in the brain. HI did not increase oxidative stress in the lungs. In the lungs, WAY100635 blunted the beneficial effects of CBD on histological damage, IL-1 concentration, and EVLW.ConclusionsCBD reduced brain HI-induced distant lung damage, with 5-HT receptor involvement in these effects. Whether the effects of CBD on the lungs were due to the anti-inflammatory effects on the brain or due to the direct effects on the lungs remains to be elucidated.

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Section: Discussionmentioning

confidence: 97%

Cannabidiol reduces lung injury induced by hypoxic–ischemic brain damage in newborn piglets

et al. 2017

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“…Cells were pre-incubated for 30 min with CBD 10 or 25 µM (working concentration previously published (Holland et al, 2006; Ligresti et al, 2006; Zhu et al, 2006; Holland et al, 2007; Holland, Allen & Arnold, 2008; De Filippis et al, 2011; Arnold et al, 2012; Harvey et al, 2012; Hill et al, 2012; Maor et al, 2012; Solinas et al, 2012; Dudášová et al, 2013; Juknat et al, 2013; Nabissi et al, 2013), and initially dissolved in DMSO) or CsA 20 µM (known P-gp inhibitor used as positive control (Mori et al, 2012)), dissolved in transport buffer (TB) (pH = 7.4) (Feinshtein et al, 2010), while “control cells” were pre-incubated in TB with the correlating concentration of DMSO. Following pre-incubation, P-gp substrates were added (Calcein-AM or DiOC2(3) or rh123) (Minderman et al, 1996; Martin et al, 2003) and cells were further incubated for 30 min.…”

Section: Immunocytochemistry and Confocal Microscopymentioning

confidence: 99%

Cannabidiol changes P-gp and BCRP expression in trophoblast cell lines

Feinshtein

Erez

Ben‐Zvi

et al. 2013

PeerJ

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Objectives. Marijuana is the most commonly used illicit drug during pregnancy. Due to high lipophilicity, cannabinoids can easily penetrate physiological barriers like the human placenta and jeopardize the developing fetus. We evaluated the impact of cannabidiol (CBD), a major non-psychoactive cannabinoid, on P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP) expression, and P-gp function in a placental model, BeWo and Jar choriocarcinoma cell lines (using P-gp induced MCF7 cells (MCF7/P-gp) for comparison).Study design. Following the establishment of the basal expression of these transporters in the membrane fraction of all three cell lines, P-gp and BCRP protein and mRNA levels were determined following chronic (24–72 h) exposure to CBD, by Western Blot and qPCR. CBD impact on P-gp efflux function was examined by uptake of specific P-gp fluorescent substrates (calcein-AM, DiOC2(3) and rhodamine123(rh123)). Cyclosporine A (CsA) served as a positive control.Results. Chronic exposure to CBD resulted in significant changes in the protein and mRNA levels of both transporters. While P-gp was down-regulated, BCRP levels were up-regulated in the choriocarcinoma cell lines. CBD had a remarkably different influence on P-gp and BCRP expression in MCF7/P-gp cells, demonstrating that these are cell type specific effects. P-gp dependent efflux (of calcein, DiOC2(3) and rh123) was inhibited upon short-term exposure to CBD.Conclusions. Our study shows that CBD might alter P-gp and BCRP expression in the human placenta, and inhibit P-gp efflux function. We conclude that marijuana use during pregnancy may reduce placental protective functions and change its morphological and physiological characteristics.

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“…Ribeiro et al, have demonstrated in two different studies that cannabidiol can decrease inflammation and improve lung functions in LPS-induced acute lung injury in mice [101,102], which is further confirmed by Vuolo et al [103]. The influence of cannabidiol on the antigen-induced contraction of guinea-pig airways has also been demonstrated [104]. However, it should also be noted that cannabidiol can cause drug interactions, hepatic abnormalities, diarrhea, fatigue, vomiting, and somnolence [105].…”

Section: Airway Diseasesmentioning

confidence: 86%

“…Therefore, blocking the CB 2 receptors in allergic airway inflammation may modulate NK cell response during airway inflammation. The plant-derived cannabinoid, cannabidiol, was also shown to inhibit allergen-induced contraction of airway smooth muscle, and reduce antigen-induced airway obstruction in guinea pigs [104]. This effect seems to be partly mediated by the inhibition of mast cell degranulation.…”

Section: Targeting the Endocannabinoid System For The Treatment Of Aimentioning

confidence: 96%

“…Due to the immunomodulatory effect of cannabinoids, a significant variety of studies were focused on their possible therapeutic potential on inflammatory diseases like asthma [43,67,68,92,93,104,[109][110][111][112][113][114]. The molecular mechanisms mediating the effects of cannabinoids in allergic airway responses mainly depend on their effects on immune cells and the related release of cytokines [115].…”

Section: Targeting the Endocannabinoid System For The Treatment Of Aimentioning

confidence: 99%

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Endocannabinoid System in the Airways

Bozkurt

2019

Molecules

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Cannabinoids and the mammalian endocannabinoid system is an important research area of interest and attracted many researchers because of their widespread biological effects. The significant immune-modulatory role of cannabinoids has suggested their therapeutic use in several inflammatory conditions. Airways are prone to environmental irritants and stimulants, and increased inflammation is an important process in most of the respiratory diseases. Therefore, the main strategies for treating airway diseases are suppression of inflammation and producing bronchodilation. The ability of cannabinoids to induce bronchodilation and modify inflammation indicates their importance for airway physiology and pathologies. In this review, the contribution of cannabinoids and the endocannabinoid system in the airways are discussed, and the existing data for their therapeutic use in airway diseases are presented.

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The effects of cannabidiol on the antigen-induced contraction of airways smooth muscle in the guinea-pig

Cited by 19 publications

References 31 publications

Cannabidiol reduces lung injury induced by hypoxic–ischemic brain damage in newborn piglets

Cannabidiol reduces lung injury induced by hypoxic–ischemic brain damage in newborn piglets

Cannabidiol changes P-gp and BCRP expression in trophoblast cell lines

Endocannabinoid System in the Airways

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