The Effects of Catabolite Derepression on the Accumulation of Steryl Esters and the Activity of  -Hydroxymethylglutaryl-CoA Reductase in Saccharomyces cerevisiae

Quain, David E.; Haslam, Jonathan

doi:10.1099/00221287-111-2-343

Cited by 48 publications

(19 citation statements)

References 26 publications

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“…Growth media and the yeast strain, ABl, were as described previously (Quain & Haslam, 1979). Yeast (10 mg dry wt) was inoculated into 1 1 glucose (1 %, w/v) medium and grown aerobically at 30 "C on a rotary shaker (180 rev.…”

Section: Methodsmentioning

confidence: 99%

“…Carbohydrates were fractionated according to the method of Trevelyan & Harrison (1956) as reported by Stewart (1975), and fractions were analysed in triplicate using the anthrone procedure (Stewart, 1975). Glucose and dry weight were determined as described previously (Quain & Haslam, 1979).…”

Section: Methodsmentioning

confidence: 99%

“…To facilitate rapid sampling with the minimum of metabolic perturbation, a modular fermentation system was used (Quain & Haslam, 1979) with an agitation rate of 300 rev. min-1 and aeration at 0.33 culture vol.…”

Section: Short Communicationmentioning

confidence: 99%

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Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Quain¹,

Haslam²

1979

Journal of General Microbiology

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Changes in the cellular content of glucose 6-phosphate and storage carbohydrates during cataboli te derepression were measured during the diauxic growth of Saccharornyces cerevisiae on glucose (1 %, w/v) medium. After the exhaustion of glucose there were transient increases in glucose 6-phosphate, alkali-soluble glycogen and trehalose, reflecting enhanced gluconeogenesis, followed by an increase in acid-soluble glycogen. During the subsequent adaptation to growth on ethanol the amounts of glucose 6-phosphate, trehalose and glycogen returned to basal levels. Thus storage carbohydrates, of which glycogen is quantitatively the most important, appear to act as the energy source for catabolite derepression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Quain¹,

Haslam²

1979

Journal of General Microbiology

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“…In many organisms, sterol biosynthesis is controlled in part by regulating the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, which catalyzes the rate-limiting step in sterol biosynthesis (reviewed in reference 8). HMGCoA reductase activity has been reported to be higher in aerobically grown cells than in anaerobically grown cells (7,43). Studies with the yeast S. cerevisiae found HMG-CoA reductase activity to be lower in heme-deficient cells than in heme-sufficient cells, suggesting that heme acts as a positive regulator of HMG-CoA reductase (27).…”

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confidence: 99%

Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.

et al. 1989

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Responses of the yeast genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, HMGI and HMG2, to in vivo changes in heme concentrations were investigated. Expression of the genes was determined by direct measurement of the mRNA transcribed from each gene, by direct assay of the enzyme activity encoded by each gene, and by measurement of the expression of lacZ fusions to the control regions of each gene. These studies indicated that expression of HMGI was stimulated by heme, whereas expression of HMG2 was repressed by heme. The effect of heme on HMG1 expression was mediated by the HAPI transcriptional regulator and was independent of HAP2. Thus, the genes encoding the 3-hydroxy-3-methylglutaryl coenzyme A reductase isozymes join a growing list of gene pairs that are regulated by heme in opposite ways.

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“…The total microsomal -hydroxy--methylglutaryl coenzyme(HMG-CoA) reductase activity was measured by the method described in Quin and Haslam (1979). Microsomes were prepared as described in .…”

Section: Enzyme Assaysmentioning

confidence: 99%

The Biological Effects of β-Cyclodextrin on Antithrombotic Activity and Plasma Lipid Metabolism in Rats

2003

Journal of Animal Science and Technology

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The effect of feeding a cyclic oligosaccharide, -cyclodextrin( CD) on plasma cholesterol and triacylglyceride concentrations and on antithrombotic activity were investigated in rats fed a control chow diet, or one either high in cholesterol or in saturated fat. The bleeding time of CD-fed groups was significantly prolonged by 293%, 157% and 218% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The whole blood clotting time was significantly increased by 202%, 168% and 211% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The CD diet caused a marked decrease in plasma total lipid (TL), triacylglyceride (TAG), total cholesterol (TC) and low density lipoproteincholesterol (LDL-C) concentrations. The plasma TL concentration was significantly decreased by 70%, 82% and 87% in normal, high cholesterol and high fat diet fed groups as compared to the control group, respectively(p<0.05). The plasma TAG concentration was significantly decreased by 89%, 43% and 59% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The plasma TC concentration was significantly decreased by 28%, 62% and 36% in normal, high cholesterol and high fat diet fed groups, respectively, as compared to the control group(p<0.05). The LDL-C concentration was significantly decreased by 39%, 54% and 25% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The plasma total bile acids contents of CD group was significantly increased by 66%, 95% and 97% in normal, high cholesterol and high fat diet fed groups as compared to control group, respectively(p<0.05). The hepatic HMG-CoA reductase activity was significantly lowered by 41% in the CD-fed group compared to normal diet fed rats(p<0.05). The fecal steroid excretions of the CD groups was significantly increased by 167% in normal diet fed rats(p<0.05). These results suggest that the CD has a biological active function on antithrombotic activity and is hypolipidemic, hypotriglyceridemic and hypocholesterolimic agents. These are all effects that can help to prevent obesity and coronary heart disease in humans.

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The Effects of Catabolite Derepression on the Accumulation of Steryl Esters and the Activity of -Hydroxymethylglutaryl-CoA Reductase in Saccharomyces cerevisiae

Cited by 48 publications

References 26 publications

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Changes in Glucose 6-Phosphate and Storage Carbohydrates during Catabolite Derepression in Saccharomyces cerevisiae

Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.

The Biological Effects of β-Cyclodextrin on Antithrombotic Activity and Plasma Lipid Metabolism in Rats

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