2019
DOI: 10.1097/ta.0000000000002225
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The effects of cell type and culture condition on the procoagulant activity of human mesenchymal stromal cell-derived extracellular vesicles

Abstract: BACKGROUND Mesenchymal stromal cell (MSC)-derived extracellular vesicles (EVs) have great potential as a cell-free therapy in wound healing applications. Because EV populations are not equivalent, rigorous characterization is needed before clinical use. Although there has been much focus on their RNA composition and regenerative capabilities, relatively less is known regarding the effects of MSC cell type (adipose tissue [Ad-MSCs] or bone marrow [BM-MSCs]) and culture condition (monolayer or sphero… Show more

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Cited by 46 publications
(58 citation statements)
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“…Our data further con rm that exosomes play a role in immunity primarily by the regulation of leukocyte-mediated immunity. In addition, based on GO analysis, AT-MSC exo may facilitate clotting, which is consistent with the ndings of Chance et al [43]. However, the clinical application for this aspect is still rare.…”
Section: Discussionsupporting
confidence: 87%
“…Our data further con rm that exosomes play a role in immunity primarily by the regulation of leukocyte-mediated immunity. In addition, based on GO analysis, AT-MSC exo may facilitate clotting, which is consistent with the ndings of Chance et al [43]. However, the clinical application for this aspect is still rare.…”
Section: Discussionsupporting
confidence: 87%
“…This different ability of EVs derived from platelets and unstimulated monocyte-like cells to support coagulation under conditions allowing for contact activation led us to hypothesize that EVs from different cell types or from different environments, such as blood vs. cell culture settings, differ regarding their procoagulant characteristics. In support of this hypothesis, recent evidence from studies on human mesenchymal stromal cellderived extracellular vesicles suggests that the ability of EVs to trigger coagulation depends on the type and state of their parent cells (Christy et al, 2017;Chance et al, 2019).…”
Section: Introductionmentioning
confidence: 91%
“…Hours after conventional IV infusion, MSCs cannot be found in circulation [13][14][15][16] . In addition, different infused MSC products have been shown to display varying levels of procoagulant activity (via the expression of tissue factor) resulting in an instant innate immune attack that can compromise the safety and efficacy of the MSCs [17][18][19] . With these issues in mind, we have been developing an engineered approach to deliver MSC therapy, where the MSCs are inoculated in an ex vivo system, allowing the cells and the blood to communicate bi-directionally through distinct compartments 20 .…”
mentioning
confidence: 99%