The effects of cholinergic and serotoninergic drugs on motility <i>in vitro</i> of <i>Haplometra cylindracea</i> (Trematoda: Digenea)

McKay, Derek M.; Halton, D W; Allen, J. M.; Fairweather, Ian

doi:10.1017/s0031182000058698

Cited by 29 publications

(8 citation statements)

References 42 publications

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“…The same inhibitory effect was observed when the worms or muscle strips were treated with classical cholinergic agonists or AChE inhibitors, whereas antagonists generally increased motor activity Sukhdeo et al 1984 ;Sukhdeo, Sangster & Mettrick, 1986 ;Thompson, Sangster & Mettrick, 1986 ;Maule et al 1989 ;McKay et al 1989). These observations led to the identification of ACh as an inhibitory neuromuscular transmitter of flatworms.…”

Section: Neuromuscular Effects Of Ach In Parasitic Flatwormsmentioning

confidence: 61%

Classical transmitters and their receptors in flatworms

2006

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The flatworm nervous system employs a wide repertoire of neuroactive substances, including small chemical messengers, the so called classical transmitters, and several types of neuropeptides. A large body of research accumulated over four decades has provided a wealth of information on the tissue localization and effects of these substances, their biochemistry and, recently, their molecular modes of action in all major classes of flatworms. This evidence will be reviewed, with particular emphasis on the small (classical) transmitters and the receptors that mediate their effects. One of the themes that will emerge from this discussion is that classical transmitters regulate core activities such as movement, metabolism and transport, and thus are essential for survival of the organism. In addition, the evidence shows that flatworms have multiple neurotransmitter receptors, many with unusual pharmacological features, which make them particularly attractive as drug targets. Understanding the molecular basis of these distinctive properties, and developing new, more specific receptor agonists and antagonists will undoubtedly become a major challenge in future research.

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Section: Neuromuscular Effects Of Ach In Parasitic Flatwormsmentioning

confidence: 61%

Classical transmitters and their receptors in flatworms

2006

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“…There have been no reports of pharmacological studies on the effects of 5-HT on digenean cercariae, presumably because of the difficulties involved with working on such small preparations. Previous studies of adult digeneans have demonstrated a likely excitatory role for 5-HT on muscle (Beretta, 1969;Fretterer et al 1977;Holmes & Fairweather, 1984;Pax et al 1984;McKay et al, 1989) and it might be predicted that 5-HT has a similar excitatory role on cercarial muscle. If proven, 5-HT could be important in motility, host location and subsequent host penetration by S. inermis cercariae.…”

Section: Discussionmentioning

confidence: 93%

The distribution of neuroactive substances within the cercaria ofSanguinicola inermis

1996

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The serotoninergic and peptidergic components of the nervous system of the cercaria of Sanguinicola inermis (Digenea: Sanguinicolidae) were examined using whole-mount immunocytochemistry and a plan of the nervous system has been described. Antibodies to serotonin (5-hydroxytryptamine, 5-HT) and the neuropeptides, FMRFamide, GFNSALMFamide (S1) and SGPYSFNSGLTFamide (S2) were used in the study. Immunoreactivity (IR) was demonstrated to all but the S2 antisera and showed a similar fundamental distribution. IR was found in paired cerebral ganglia located anteriorly within the body and connected by a cerebral commissure. From the ganglia paired ventral and dorsal longitudinal nerve cords extend anteriorly into the cephalic organ and into the body. There is no apparent connection with the tail. Several transverse commissures connect the longitudinal nerve cords throughout the body and several associated cell bodies have been located. A double-stranded dorsal and ventral longitudinal nerve cord extends the length of the tail and six cell bodies are associated with these cords, uniquely demonstrating either FMRFamide and S1, or 5-HT-like IR. Only 5-HT-like IR was found to extend into the posterior tail furcae and there appears to be a lack of any peripheral tegumental innervation. Double-labelling experiments suggest that the serotoninergic and peptidergic components of the cercarial nervous system are distinct.

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“…Immunocytochemical data also indicates the presence of 5‐HT in the nervous system of trematodes (Barton, Halton, Shaw, Maule, & Johnston, 1993; Fairweather, Maule, Mitchell, & Halton, 1987; Magee, Cahir, Halton, Johnston, & Shaw, 1993; McKay et al, 1991; Terenina et al, 2020; Tolstenkov et al, 2010). It is assumed that 5‐HT plays a role as an excitatory neurotransmitter in flatworms inducing muscle contraction (El‐Shehabi, Vermeire, Yoshino, & Ribeiro, 2009; McKay, Halton, Allen, & Fairweather, 1989; Moneypenny et al, 2001; Patocka, Sharma, Rashid, & Ribeiro, 2014; Pax, Siefker, & Bennett, 1984; Thompson & Mettrick, 1989; Zamanian et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

The neuroactive substances and associated muscle system in Rhipidocotyle campanula (Digenea, Bucephalidae) from the intestine of the pike Esox lucius

Kreshchenko

Теренина

Nefedova

et al. 2020

Journal of Morphology

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We report about the muscular system and the serotonergic and FMRFamidergic components of the nervous system of the Bucephalidae trematode, Rhipidocotyle campanula, an intestinal parasite of the pike. We use immunocytochemical methods and confocal scanning laser microscopy (CLSM). The musculature is identified by histochemical staining with fluorescently labeled phalloidin. The body wall musculature of R. campanula contains three layers of muscle fibresthe outer thin circular, intermediate longitudinal and inner diagonal muscle fibres running in two opposite directions. The digestive system of R. campanula possess of a well-developed musculature: radial, longitudinal and circular muscle elements are detected in the pharynx, circular and longitudinal muscle filaments seen in the oesophagus, and longitudinal and the circular muscle fibres were found in the intestinal wall. Specific staining indicating the presence of actin muscle filaments occurs in the cirrus sac localized in the posterior body region. The frontal region of anterior attachment organ, the rhynchus, in R. campanula is represented by radial muscle fibres. The posterior part of the rhynchus comprise of radial muscles forming the organ's wall, and several strong longitudinal muscle bundles. Serotonergic and FMRFamidergic structures are detected in the central and peripheral compartments of the nervous system of R. campanula, that is, in the paired brain ganglia, the brain commissure, the longitudinal nerve cords, and connective nerve commissures. The innervations of the rhynchus, pharynx, oesophagus and distal regions of the reproductive system by the serotonergic and FMRFamidergic nervous elements are revealed. We compare our findings obtained on R. campanula with related data for other trematodes.

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The effects of cholinergic and serotoninergic drugs on motility in vitro of Haplometra cylindracea (Trematoda: Digenea)

Cited by 29 publications

References 42 publications

Classical transmitters and their receptors in flatworms

Classical transmitters and their receptors in flatworms

The distribution of neuroactive substances within the cercaria ofSanguinicola inermis

The neuroactive substances and associated muscle system in Rhipidocotyle campanula (Digenea, Bucephalidae) from the intestine of the pike Esox lucius

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