The Effects of Chronic Hyperinsulinaemia on Insulin Binding and Glucose Metabolism in Rat Adipocytes

Whittaker, Jonathan; Kg, Alberti; Da, York; Singh, Jaspal

doi:10.1042/bst0071055

Cited by 19 publications

(16 citation statements)

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“…We found that continuous hyperinsulinemia for either 2 or 6 wk did not result in the development of insulin resistance in either adipose cells or skeletal muscle. On the contrary, after 2 wk of insulin administration, insulin binding to adipose cells was increased rather than decreased, as has been reported previously by Kobayashi and Olefsky (14) and Whittaker et al (15), and nearly twofold increases in total insulin-stimulated glucose metabolism by adipose cells were observed at insulin concentrations > 10 ,gU/ml. In addition, we have extended the period of hyperinsulinemia to 6 wk, following which insulin binding to adipose cells and insulin-stimulated glucose metabolism remained increased.…”

Section: Discussionsupporting

confidence: 61%

“…After 6 wk of treatment, the mean daily consumption of chow and sucrose was the same ih insulin-treated animals and the sugar-supplemented controls (chow: 15 …”

Section: Resultsmentioning

confidence: 92%

“…In addition, the capacity for intracellular glucose metabolism was increased (17). In contrast, Whittaker et al (15) found no change in maximal insulin-stimulated glucose transport, although they did confirm a rightward shift in the insulin doseresponse curve and an increase in insulin-stimulated glucose metabolism. These results suggest that chronic hyperinsulinemia has different long-term effects on the down-regulation of the insulin receptor and on glucose transport and metabolism.…”

Section: Introductionmentioning

confidence: 75%

“…Recently, Kobayashi and Olefsky (14) and Whittaker et al (15) have demonstrated that chronic hyperinsulinemia, induced by daily injections of insulin, is associated with a reduced concentration of insulin receptors in adipose cells and a decrease in cellular insulin sensitivity consistent with the concept of down-regulation of the insulin receptor. However, Kobayashi and Olefsky (14,16) have also reported that chronic hyperinsulinemia has significant postreceptor effects on glucose transport and metabolism.…”

Section: Introductionmentioning

confidence: 86%

“…Previous studies have examined the effects of experimental chronic hyperinsulinemia on glucose transport and metabolism and insulin binding in adipose cells (14)(15)(16)(17). In these studies, the hyperinsulinemia was induced by injection of NPH or Ultratard insulin over a period of -2 wk.…”

Section: Discussionmentioning

confidence: 99%

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Regulation of glucose utilization in adipose cells and muscle after long-term experimental hyperinsulinemia in rats.

Wardzala¹,

Hirshman²,

Pofcher³

et al. 1985

J. Clin. Invest.

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The effects of chronic insulin administration on the metabolism of isolated adipose cells and muscle were studied. Adipose cells from 2 and 6 wk insulin-treated and control rats, fed either chow or chow plus sucrose, were prepared, and insulin binding, 3-0-methylglucose transport, glucose metabolism, and lipolysis were measured at various insulin concentrations. After 2 wk of treatment, adipose cell size and basal glucose transport and metabolism were unaltered, but insulin-stimulated transport and glucose metabolism were increased two-to threefold when cells were incubated in either 0.1 mM glucose (transport rate limiting) or 10 mM glucose (maximum glucose metabolism). Insulin binding was increased by 30%, but no shift in the insulin dose-response curve for transport or metabolism occurred. After 6 wk of treatment, the effects of hyperinsulinemia on insulin binding and glucose metabolism persisted and were superimposed on the changes in cell function that occurred with increasing cell size in aging rats. Hyperinsulinemia for 2 or 6 wk did not alter basal or epinephrine-stimulated lipolysis in adipose cells or the antilipolytic effect of insulin. In incubated soleus muscle strips, insulin-stimulated glucose metabolism was significantly increased after 2 wk of hyperinsulinemia, but these increases were not observed after 6 wk of treatment. We conclude that 2 wk of continuous hyperinsulinemia results in increased insulin-stimulated glucose metabolism in both adipose cells and soleus muscle. Despite increased insulin binding to adipose cells, no changes in insulin sensitivity were observed in adipose cells or muscle. In adipose cells, the increased glucose utilization resulted from both increased transport (2 wk only) and intracellular glucose metabolism (2 and 6 wk). In muscle, after 2 wk of treatment, both glycogen synthesis and total glucose metabolism were increased. These effects of hyperinsulinemia were lost in muscle after 6 wk of treatment, when compared with sucrose-supplemented controls.

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Section: Discussionsupporting

confidence: 61%

“…After 6 wk of treatment, the mean daily consumption of chow and sucrose was the same ih insulin-treated animals and the sugar-supplemented controls (chow: 15 …”

Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation of glucose utilization in adipose cells and muscle after long-term experimental hyperinsulinemia in rats.

Wardzala¹,

Hirshman²,

Pofcher³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Isolated adipocytes: An assessment of cell surface changes during their preparation

Al‐Jafari

Lee²,

Tume

et al. 1986

Cell Biochemistry & Function

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A method is described, based on the detection of adipocyte-specific cell surface antigens, which allows assessment of the relative surface damage incurred by the cells when they are prepared under a variety of conditions. Using the method it is possible to develop, for any set of reagents, a set of cell isolation conditions (collagenase concentration, time of incubation) which will produce minimally damaged cells which exhibit high levels of specific cell surface immunoreactivity. Under certain conditions a recovery from limited surface damage can be achieved, although, when cells are prepared under more extreme conditions irreversible surface damage occurs. The surface morphology of the cells as revealed by scanning electron microscopy, is also clearly affected by the conditions of cell isolation. The method has been used to define the conditions necessary for the isolation of cells to be used in the study of subtle biochemical responses.

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Subcellular translocation of glucose transporters: Role in insulin action and its perturbation in altered metabolic states

Kahn

Cushman

1985

Diabetes Metab. Rev.

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The Effects of Chronic Hyperinsulinaemia on Insulin Binding and Glucose Metabolism in Rat Adipocytes

Cited by 19 publications

References 0 publications

Regulation of glucose utilization in adipose cells and muscle after long-term experimental hyperinsulinemia in rats.

Regulation of glucose utilization in adipose cells and muscle after long-term experimental hyperinsulinemia in rats.

Isolated adipocytes: An assessment of cell surface changes during their preparation

Subcellular translocation of glucose transporters: Role in insulin action and its perturbation in altered metabolic states

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