The Effects of Chunghyul‐Dan, an Agent of Korean Medicine, on a Mouse Model of Traumatic Brain Injury

Choi, Won Hoon; Lee, Kyungjin; Lee, Beomjoon; Park, Seong-Uk; Park, Jung-Mi; Ko, Chang‐Nam; Bu, Youngmin

doi:10.1155/2017/7326107

Cited by 6 publications

(8 citation statements)

References 53 publications

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“…Together with CAPE, they exhibited potent anti-proliferative and proapoptotic properties. Currently, CAPE is one of the most promising compounds [ 4 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and Biological Evaluation of Propolis from Poland

et al. 2017

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In this study, we assessed the therapeutic potential of propolis from Poland and performed chemical analysis by GC–MS, as well as determined its botanical origin. Chemical constituents typical for bud exudates of Populus nigra (section Aigeiros) were determined, however, glycerol esters of phenolic acids, as well as unusually high amounts of p-coumaric and ferulic acid and their benzyl esters, were also detected. These constituents are characteristic for buds of Populus tremula (section Leuce). We also evaluated the antiproliferative effect of propolis extracts against nine human cancer cell lines. Additionally, promising antibacterial activity of the dichloromethane extract (Minimal Inhibitory Concentration MIC values of 0.95–1.24 mg/mL), as well as a moderate antifungal activity (MIC values of 1.25–1.40 mg/mL), was noticed. Propolis from Poland appeared as a rich source of antibacterial and antiproliferative compounds and this confirmed that it is a valuable natural product with the potential to improve human health.

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“…Together with CAPE, they exhibited potent anti-proliferative and proapoptotic properties. Currently, CAPE is one of the most promising compounds [ 4 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and Biological Evaluation of Propolis from Poland

et al. 2017

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“…Other doses, 3 and 30 mg/kg, were selected at three time intervals (low dose and high dose). The positive control condition used in the current study, minocycline, has been reported to have protective effects against various brain injuries including TBI [36,37]. The dose of 45 mg/kg was chosen based on a previous report [36].…”

Section: Discussionmentioning

confidence: 99%

“…The TBI model was induced according to the modified CCI protocol [36]. Briefly, ICR mice were anesthetized with isoflurane (2 %).…”

Section: Tbi Inductionmentioning

confidence: 99%

“…Mice were randomly divided into six groups: sham group, control group, minocycline-treated group, and icariin (CAS 489-32-7, purity 96 %; Tokyo Chemical Industry Co., Ltd) 3, 10, and 30 mg/ kg-treated group (n = 8). Icariin was dissolved in distilled water and administered orally with a volume of 3.3 mL/kg simultaneously with TBI induction at first and then continued twice per day for 7 d. Minocycline (45 mg/kg) was dissolved in normal saline and injected intraperitoneally [36,38]. Control group were given the same volume of distilled water.…”

Section: Grouping and Drug Administrationmentioning

confidence: 99%

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Icariin Improves Functional Behavior in a Mouse Model of Traumatic Brain Injury and Promotes Synaptic Plasticity Markers

et al. 2018

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Epimedii Herba (EH) has been used in traditional Asian medicine to treat hemiplegia following stroke. Icariin, its major active component, is used as a quality-control marker and for its various pharmacological effects. We hypothesized that icariin would show protective effects following traumatic brain injury (TBI). The TBI mouse model was induced using a controlled cortical impact method. Body weight, brain damage, motor function, and cognitive function were evaluated. Synaptogenesis markers were analyzed to investigate potential mechanisms of action. The animals were divided into six groups: sham, control, minocycline-treated group, and icariin-treated (3, 10, and 30 mg/kg, p. o.) groups. The icariin 30 mg/kg-treated group regained body weight at 7 and 8 d post TBI. Icariin 30 mg/kg- and 10 mg/kg-treated groups showed enhanced sensory-motor function at 8 d post TBI in rotarod and balance beam tests. Icariin-treated groups showed increased recognition index in the novel object recognition test at all doses and increased spontaneous alternation in the Y-maze test at 30 mg/kg. Icariin upregulated brain-derived neurotrophic factor, synaptophysin and postsynaptic density protein 95 expressions. However, no protective effects against brain damage or neuronal death were observed. The current results provide a basis for using icariin following TBI and suggest that it could be a candidate for the development of therapeutic agents for functional recovery after TBI.

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“…Mice were randomly assigned to each experimental groups. In vivo model of TBI was induced using the CCI method 32,33 . Briefly, ICR mice (8 weeks old, male, 30–33 g) were anesthetized with 2% isoflurane in gas mixture of 70% N 2 O and 30% O 2 , and placed in the stereotaxic frame.…”

Section: Methodsmentioning

confidence: 99%

Brain injury induces HIF-1α-dependent transcriptional activation of LRRK2 that exacerbates brain damage

et al. 2018

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Leucine-rich repeat kinase 2 (LRRK2), originally identified as a causative genetic factor in Parkinson’s disease, is now associated with a number of pathologies. Here, we show that brain injury induces a robust expression of endogenous LRRK2 and suggest a role of LRRK2 after injury. We found that various in vitro and in vivo models of traumatic brain injury (TBI) markedly enhanced LRRK2 expression in neurons and also increased the level of hypoxia-inducible factor (HIF)-1α. Luciferase reporter assay and chromatin immunoprecipitation revealed direct binding of HIF-1α in LRRK2 proximal promoter. We also found that HIF-1α-dependent transcriptional induction of LRRK2 exacerbated neuronal cell death following injury. Furthermore, application of G1023, a specific, brain-permeable inhibitor of LRRK2, substantially prevented brain tissue damage, cell death, and inflammatory response and alleviated motor and cognitive defects induced by controlled cortical impact injury. Together, these results suggest HIF-1α-LRRK2 axis as a potential therapeutic target for brain injury.

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The Effects of Chunghyul‐Dan, an Agent of Korean Medicine, on a Mouse Model of Traumatic Brain Injury

Cited by 6 publications

References 53 publications

Characterization and Biological Evaluation of Propolis from Poland

Characterization and Biological Evaluation of Propolis from Poland

Icariin Improves Functional Behavior in a Mouse Model of Traumatic Brain Injury and Promotes Synaptic Plasticity Markers

Brain injury induces HIF-1α-dependent transcriptional activation of LRRK2 that exacerbates brain damage

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