Due to the COVID-19 pandemic, there has been a significant shift from traditional, in-person teaching to online education, which has become indispensable. Nowadays, online learning is increasingly popular, encompassing educational programs delivered exclusively through digital platforms like video conferences and virtual classrooms. The flexibility of online education allows students to learn at their own pace and according to their schedule, but it also poses potential disadvantages. In this article, we explore the impact of online teaching on the practical subject of biology and students’ perceptions of education in primary, secondary, and tertiary schools in Slovakia. Our research focuses on evaluating the progress of online biology teaching, examining methods of knowledge testing, and assessing both the positives and negatives.