The effects of culture concentration and age, time, and temperature on bacterial attachment to polystyrene

Abstract

The attachment of a marine pseudomonad to polystyrene was found to be dependent upon (a) culture concentration, (b) the time allowed for attachment, (c) the growth phase of the culture, and (d) temperature. The number of attached cells increased with both culture concentration and the time allowed for attachment, until the attachment surface approached saturation. The number of cells which attached and the rate of attachment was greatest with log-phase cultures, and progressively decreased with stationary and death-phase cultures. A temperature of 3 °C ± 1 noticeably decreased the proportion of stationary phase cells which attached, compared with cells at 20 °C ± 1. These results can be described by a model based on physicochemical adsorption, which suggests that non-biological processes may play a major role in the initial events of bacterial adhesion.

Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis

“…The initiation of biofilm formation was assayed by the ability of P. fluorescens to attach to the wells of microtitre dishes made of polyvinylchloride (PVC), using a modification of a previously described assay (Fletcher, 1977). The biofilm was detected by staining with crystal violet (CV).…”

“…This assay is based on the ability of bacteria to form biofilms on polyvinylchloride plastic (PVC), a material used to make catheter lines (Lopez-Lopez et al, 1991). Biofilm formation was assayed by the ability of cells to adhere to the wells of 96-well microtitre dishes made of PVC (Falcon 3911 Microtest III flexible assay plate; Becton Dickinson Labware) using a modification of a previously reported protocol (Fletcher, 1977). The indicated medium (100 l/well) was inoculated either from cells patched on LB agar plates using a multiprong device or from a 1:100 dilution from an overnight LB culture.…”

IS5 inserts upstream of the master motility operon flhDC in a quasi-Lamarckian way

“…Crystal violet biofilm assay Biofilm formation was assayed in 96-well polystyrene plates using 0.1% crystal violet staining (Corning Costar, Cambridge, MA, USA) (Fletcher, 1977). Briefly, each well was inoculated at an initial turbidity at 600 nm of 0.05 and grown without shaking for 8 h in LB and M9C medium.…”

Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chambe in the absence and presence of nutrient broth

“…1989), cell-surface hydrophobicity (Ljungh 8c Wadstrom, 1995) and adhesion (Fletcher, 1976;Zeng et al, 1994), depending upon growth conditions. Alternatively, adhesion studies sometimes involve optimal growth conditions.…”